Vehicle safety risk can be decreased by diagnosing the lithium-ion battery system of electric road vehicles. Real-time cell diagnostics can avoid unexpected occurrences. However, lithium-ion batteries in electric vehicles can significantly differ in design, capacity, and chemical composition. In addition, the battery monitoring systems of the various vehicles are also diverse, so communication across the board is not available or can only be achieved with significant difficulty. Hence, unique type-dependent data queries and filtering are necessary in most cases. In this paper, a Volkswagen e-Golf electric vehicle is investigated; communication with the vehicle was implemented via an onboard diagnostic port (so-called OBD), and the data stream was recorded. The goal of the research is principally to filter out, identify, and localize defective/weak battery cells. Numerous test cycles (constant and dynamic measurements) were carried out to identify cell abnormalities (so-called deviations). A query and data filtering process was designed to detect defective battery cells. The fault detection procedure is based on several cell voltage interruptions at various loading levels. The methodology demonstrated in this article uses a fault diagnosis technique based on voltage abnormalities. In addition, it employs a hybrid algorithm that executes calculations on measurement and recorded data. In the evaluation, a status line comprising three different categories was obtained by parametrizing and prioritizing (weighting) the individual measured values. It allows the cells to be divided into the categories green (adequate region), yellow (to be monitored), and red (possible error). In addition, several querying strategies were developed accordingly to clarify and validate the measurement results. The several strategies were examined individually and analyzed for their strengths and weaknesses. Based on the results, a data collection, processing, and evaluation strategy for an electric vehicle battery system have been developed. The advantage of the developed algorithm is that the method can be adapted to any electric or hybrid vehicle battery.