The plasma jet has been proposed as a novel therapeutic method for anticancer treatment. However, its biological effects and mechanism of action remain elusive. Here, we investigated its cell death effects and underlying molecular mechanisms, using air and N2 plasma jets from a micro nozzle array. Treatment with air or N2 plasma jets caused apoptotic death in human cervical cancer HeLa cells, simultaneously with depolarization of mitochondrial membrane potential. In addition, the plasma jets were able to generate reactive oxygen species (ROS), which function as surrogate apoptotic signals by targeting the mitochondrial membrane potential. Antioxidants or caspase inhibitors ameliorated the apoptotic cell death induced by the air and N2 plasma jets, suggesting that the plasma jet may generate ROS as a proapoptotic cue, thus initiating mitochondria-mediated apoptosis. Taken together, our data suggest the potential employment of plasma jets as a novel therapy for cancer.

This study investigated infant social looking in a social referencing procedure with mothers and strangers. Sixty-one infants and their mothers participated when the infants were 18 and 24 months old. The frequency and latency of looking toward each adult when the baby encountered remotecontrolled ambiguous toys were measured. Mothers and strangers (female experimenters) expressed positive or negative emotional responses whenever children looked toward them. Results indicated that older infants looked more often and faster, especially toward strangers. Both age groups tended to look to the strangers more frequently than to mothers. The pattern of looking toward caregivers over time differed from looking toward strangers in both age groups. These results indicate that nonattachment figures may be preferred targets of referencing in certain situations. It is also implied that older infants' looks to adults might differ from those of their younger counterparts in a number of ways.

The present study examined the role of emotion and emotion control in children's externalizing problems. Third- to sixth-grade children were administered a self-report measure of positive emotion, negative emotion, and emotion control. Peer- and teacher-reported adjustment problems were assessed. Structural equations modeling revealed that negative emotion, especially anger, was important in externalizing problems. Less positive emotion was associated with more externalizing problems. However, when negative emotion was examined in a more differentiated manner (anger, sadness and fear), the effect of positive emotion was diminished. Anger consistently emerged as a significant predictor of behavior problems. No interaction between either positive emotion and emotion control or negative emotion and emotion control was significant. Results showed main effects of each emotion component, with small interaction effects. Methodological and conceptual implications of the findings from the present study are discussed.

This study examined the relative family-wise error (FWE) rate and statistical power of multivariate permutation tests (MPTs), Bonferroni-adjusted alpha, and uncorrected-alpha tests of significance for bivariate associations. Although there are many previous applications of MPTs, this is the first to apply it to testing bivariate associations. Electrocortical studies were selected as an example class because the sample sizes that are typical of electrocortical studies published in 2001 and 2002 are small and their multiple significance tests are typically nonindependent. Because Bonferroni adjustments assume independent predictors, we expected that MPTs would be more powerful than the Bonferroni adjustment. Results support the following conclusions: (a) failure to control for multiple significance testing results in unacceptable FWE rates, (b) the FWE rate for the MPTs approximated the alpha set for the analyses, and (c) the statistical power advantage that MPTs provide over Bonferroni adjustments is important when using small sample sizes such as those that are typical of recent electrocortical studies.