Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ twelve species, while Boulos (1999) enumerated fourteen species.Genus Vicia L. contains the extensively cultivated faba bean, Vicia faba, the minor forages V. narbonensis, V. sativa subsp. sativa (common vetch) and the taxa that have a high potential for use as forages of the future: V. hyaeniscyamus, V. noeana, and V. sativa subsp. amphicarpa (Maxted et al., 1991;Maxted, 1995). The three species, V. sativa L., V. villosa Roth and V. narbonensis L., are cultivated (in Spain, Turkey, Jordan, Syria and Iraq) for their high-quality fodder and proteinrich seeds (28 to 32%) (Siddique et al., 1996;Caballero et al., 2001). Vicia sativa subsp. sativa, which is known as the common vetch, is one of the most commonly grown winter cover crop, or

PHLOMIS AUREA Decne. (Lamiaceae), which have future antidiabetic drug yielding potentials, is an endemic species, restricted to the high altitudes in five main habitats in southern Sinai. The genetic characterization of individuals from different populations is necessary to construct proper conservation programs. Thus, the present study was conducted to achieve two main goals; 1) Finding the genetic diversity among Phlomis aurea populations through variation in seed storage proteins electrophoretic pattern and Inter Simple Sequence Repeats (ISSR) finger printing as a dominant DNA molecular marker. 2) Perceive the relationship among these biochemical and molecular parameters with the different habitats in Saint Katherine Protectorate (SKP) are in South Sinai. Mature dry seeds from 30 individuals representing different populations were used for protein analysis using SDS-PAGE technique, 12 individuals of them were chosen for ISSR analysis. The former test produced 20 protein bands, three of them were common to all plants (species specific) and could be used as finger prints for Phlomis aurea. It was suggested that seed storage protein patterns are affected by aspect direction, altitude and slightly affected by habitats. ISSR analysis showed that decreasing genetic variation of Phlomis aurea individuals from different habitats in the following order: Wadibed> Slope> Gorge>Basin. This open area habitat contributes to high percent of hybridization and consequently results in high genetic polymorphism.

T HE HIGH economic importance of the genus Vicia has led to a large body of studies on the molecular characterization and investigation of phylogenetic relationships among species that belonged to this genus. The Mediterranean area is the principal center of distribution and diversification where polymorphism has been associated with the geographical origin of germplasm (ICARDA, 2013). The extent of genetic diversity in germplasm can be assessed through morphological characterization and genetic markers. The molecular techniques have a more accurate evaluation of the genetic and environmental components of variation, bringing greater precision to measures of genetic diversity. The present study is a molecular analysis of Vicia germplasm through ISSR and ITS techniques. The objectives were to determine the genetic diversity and phylogenetic relationship among defined 19 accessions, collected by ICARDA from different countries and habitats, representing nine Vicia species and subspecies: V. ervilia; V. monantha; V. villosa subsp. villosa, V. villosa subsp. dasycarpa and V. villosa subsp. eriocarpa; V. sativa subsp. nigra, subsp. amphicarpa, subsp. macrocacrpa and subsp. sativa. The patterns of variation of those accessions were studied by the numerical analysis of data to generate pertinent genetic information for the complement of passport data of ICARDA germplasm collections.

L EAF macro-and micro-characters of seven genera represented by twenty species belonging to tribe Malveae, family Malvaceae were examined, measured and investigated in this study. The obtained results indicated that the petiole length, leaf blade shape, base and apex varied between the studied taxa. Meanwhile the leaf margin, number and depth of teeth/ cm in the leaf margin, type of venation and number of lateral veins can be of diagnostic value within the species. Epidermal cell characters are mostly invariable, while the type and density of hairs and presence of cavitated hairs beside the type of stomata and shape of guard cells gave valuable characters within the taxa. The results have been subjected to clustering analyses using PAST program and the resulted dendrogram shows great similarity between the taxa with the separation of both Malva aegyptia and Althaea ludwigii each in a separate clade. Diagnostic key has been instructed and the phylogenetic thinking has been postulated.

Review History