The greenhouse experiments were carried out to study the effects foliar application of L-cysteine, L-methionine and Aminoacid fertilizer (AA) on Broccoli (Brassica oleracea var. italica) transplants. L-cysteine, L-methionine and AA was applied at three concentrations, 0, 100 and 200 mg L -1 commencing from 20 days after the sowing. Physical and chemical properties of the transplants determined according to the methods described by Tabatabaee. Results showed that L-cysteine at concentrations of 100 and 200 mg L -1 increased the leaf area by 53% and 48%, while methionine and the amino-acid-bearing fertilizer, both at 200 mg L -1 , increased the leaf area by 59% and 48% respectively. These percentages are expressed in comparison to the control. L-cysteine at concentrations of 100 and 200 mg L -1 increased the fresh weight of shoots by 50% and 55%, respectively, while methionine at 100 and 200 mg L -1 increased the fresh weight of shoots by 55% and 60% respectively. The amino-acid-bearing fertilizer treatments at a concentration of 200 mg L -1 increased the shoot dry weight of Broccoli seedlings by 38%, L-cysteine at concentrations of 100 and 200 mg L -1 increased the root dry weight by 26% and 60%, methionine at 100 and 200 mg L -1 increased the root dry weight by 31% and 177%. The results showed that methionine at 200 mg L -1 increased chlorophyll by 61%. The amino-acid-bearing fertilizer at a concentration of 200 mg L -1 managed to increase the length of transplants by 30%, compared to the control. Methionine at 100 and 200 mg.L -1 increased the amount of nitrogen in transplanted Broccoli by 36% and 38%.
A greenhouse experiment was carried out to determine the influence of replacing 80% of urea in broccoli fertilization with L-cysteine and L-methionine foliar spray at two concentrations 100 and 200 mgL-1 and amino acid containing fertilizer (AA) at 750 and 1500 mgL-1 on yield, nitrate accumulation, dry weight of head, vitamin C, phenolic compounds and total acidity. Replacing 80% of urea with AA increased the total acidity significantly (P ≤ 0.05). Elimination of 80% urea but foliar spray of cysteine (100 and 200 mgL-1), methionine (200) the percentage of dry weight significantly (P ≤ 0.05). Urea (250 kg ha-1) plus amino acids and amino acid Alone in Comparison with control (just 50 kg ha-1 urea) increased significantly (P ≤ 0.05) Yield. The content of vitamin C, decrease significantly (P ≤ 0.05) by the urea (250 kg ha-1) application with amino acids. Application of amino acid containing fertilizer (750 and 1500 mgL-1) along with urea (250 kg ha-1) increased nitrate accumulation.
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