Cellulose, a carbohydrate mainly found in plant cell walls, is most abundant biopolymer on the Earth. Biodegradation of cellulose is carried out by a group of enzymes knows as cellulases. These enzymes play important roles in food processing and used in food industry. In the present era, cold-active enzymes are preferred instead of meso- and thermos-philic counterparts due to less energy requirement for their optimal activity and easy inactivation. The present study includes evaluation of cold-active cellulase from Pseudoalteromonas haloplanktis for its industrial applications in comparison to mesophilic and thermophilic cellulases, through molecular docking method. The binding energy of cold-active cellulase with the substrate cellulose was -126.60 KCals/mole. However, the energy for thermo- and mesophilic cellulase found to be -93.29 and -75.54 KCals/mole, respectively. The results concluded that cold-active cellulase has more efficacy compared to its counterparts and may be used in food processing industry at commercial level.

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Biosensors are nowadays being used in various fields including disease diagnosis and clinical analysis. The ability to detect biomolecules associated with disease is vital not only for accurate diagnosis of disease but also for drug discovery and development. Among the different types of biosensors, electrochemical biosensor is most widely used in clinical and health care services especially in multiplex assays due to its high susceptibility, low cost and small in size. This article includes comprehensive review of biosensors in medical field with special emphasis on electrochemical biosensors for multiplex assays and in healthcare services. Also, the publications on electrochemical biosensors are increasing rapidly; therefore, it is crucial to be aware of any latest developments or trends in this field of research. We used bibliometric analyses to summarize the progress of this research area. The study includes global publication counts on electrochemical biosensors for healthcare along with various bibliometric data analyses by VOSviewer software. The study also recognizes the top authors and journals in the related area, and determines proposal for monitoring research.