Background: Vimentin is a prominent Intermediate Filaments (IFs) protein expressed in different mesenchymal origin cell types. Besides a wide range of cellular function roles associated with vimentin expression, its dysregulation and cell surface expression in the induction of malignancy properties have been reported extensively, making it a promising cancer-specific target. Therefore, this study aimed to generate and characterize anti-vimentin monoclonal antibodies. Methods: A 14-mer synthetic peptide from vimentin was conjugated to Keyhole Limpet Hemocyanin (KLH) and used for immunization of Blab/C mice and monoclonal production by conventional hybridoma technology. The monoclonal antibody was purified using affinity chromatography of supernatants from the selected hybridoma cells. ELISA, Immunoprecipitation-Western blotting (IP-WB), Immunocytochemistry (ICC), and flow cytometry were employed to characterize the produced monoclonal antibody in terms of interaction with vimentin immunizing peptide as well as vimentin protein. Results: Amid the several obtained producing anti-vimentin antibody hybridomas, the 7C11-D9 clone (IgG1 isotype with kappa light chain) showed higher reactivity with the immunizing peptide, and led to its selection for purification and characterization. The purified antibody could detect vimentin protein in IP-WB, ICC and flow cytometry of the normal and cancerous cells with different origin. No vimentin expression was found in normal healthy Peripheral Blood Mononuclear Cell (PBMC). Conclusion: Taken together, 7C11-D9 anti-vimentin monoclonal antibody might be used as immune diagnostic or immune therapeutic tool where detection or targeting of vimentin in a wide range of organisms is required.

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Dermoid cyst is a benign and common ovarian neoplasm affecting women. Sources for this review article were taken from PubMed and other up-to-date databases covering the period from Jan 2010 to Jan 2016. Keywords for the search were "dermoid cyst" and "treatment". A search of the literature revealed 113 full text manuscripts, from which 21 were relevant. In addition, another 56 relevant manuscripts identified in the reference lists of the above-mentioned 21 manuscripts were included in the study, although they had been published before 2010. Clinical considerations for dermoid cyst management are categorized as follows: 1) selection of the best choice of surgical treatment in dermoid cyst: laparoscopy or laparotomy; 2) procedure to exteriorize a dermoid cyst in laparoscopy; 3) selection of oophorectomy or cystectomy; 4) spillage of the cyst contents: prevention and treatment of the consequences if it does happen; 5) necessity of surgical treatment in dermoid cyst management; 6) ovarian torsion and other complications; 7) Probability of malignancy in dermoid cyst.

Background: Osteoporosis might result in an increased risk of bone fracture. Diagnosis of osteoporosis results in proper treatment and reduction of fracture rate. Objectives: This study aimed to construct a predictive model of osteoporosis case finding in Iranian women. Methods: A prospective diagnostic value study was designed, enrolling 317 asymptomatic women 50 years old or more referred for screening, at the Imam Hossein Medical Center, Tehran, Iran, for two years. The data was collected with the census method. A questionnaire including risk factors was completed, and bone mass densitometry (BMD) was done by the dual-energy X-ray absorptiometry (DXA) method in all cases. According to standard curves, bone density of the femur and lumbar spine clarified osteoporosis status for each person. In the first step, univariate analysis with osteoporosis as the main outcome did use the chi-squared test, independent sample t-test. In the next step, factors with a P-value of less than 0.2 were included in the multivariate logistic model, and a predictive model was constructed. The goodness of fit test was applied to assess the model building. The area under the curve (AUC) calculated for the model and the best cut-point for risk of menopause according to the Youden index were determined. The significance level was set at 0.05 for statistical analysis. Statistical analyses did use the program SSPS version 17.0 (SPSS, Inc., Chicago, IL, USA). Results: In 317 cases of the present study, the mean age of the population was 52.46 years old. Ninety-nine (%31.2) of these asymptomatic women revealed osteoporosis on the BMD test. Age and family history of osteoporosis were risk factors, and BMI, parity, and menopause age were protective factors for osteoporosis. Constructed model of osteoporosis prediction was as follows: (age × 0.149) + (family history × 0.963) - (BMI × 0.088) - (menopause age × 0.097) - (parity × 0.80). Optimal cutoff = 0.336 based on Youden method was chosen to predict the risk of osteoporosis. Conclusions: BMD test in Iran in more than 50 years old might find positive osteoporotic cases in at least 23.8%. BMD test in Iran in more than 50 years old might find positive osteoporotic cases in at least 23.8%. A model of osteoporosis probability constructed based on age, family history, menopause age, and parity in the present study can predict women at risk of osteoporosis.

Lymphedema is an unusual and specific type of peripheral edema resulting from obstruction or disruption of lymphatic system. The present review was conducted on PubMed, UpToDate, and ClinicalKey databases before 2016. The keywords included lymphedema or leg edema AND advanced malignancy. The primary review revealed 104 full text publications, of which 24 relevant articles were selected and another 17 relevant articles from the reference list of the selected articles were added, as well. Practical points in diagnosis and treatment of lymphedema in gynecologic malignancies are presented in the below subtitles: -basic descriptions, classifications, and epidemiology; -clinical presentation and diagnostic tests; -differential diagnosis; -non-surgical management; -surgical management.