Rapeseed (Brassica napus), an important oilseed crop, has adapted to diverse climate zones and latitudes by forming three main ecotype groups, namely winter, semi-winter, and spring types. However, genetic variations underlying the divergence of these ecotypes are largely unknown. Here, we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions. A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs) as well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of ''Darmor-bzh'' and ''Tapidor,'' respectively. We generated a map of allelic drift paths that shows splits and mixtures of the main populations, and revealed an asymmetric evolution of the two subgenomes of B. napus by calculating the genetic diversity and linkage disequilibrium parameters. Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses. A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups, and will facilitate screening of molecular markers for accelerating rapeseed breeding.
Largely accessible organic wastes can be turned into valuable compost product for raising crops organically on one hand, and get them disposed off safely at the other end. Straight use of organic wastes has tribulations like transportation and handling, wider C:N ratio, high application rates, nutrient overloading, weed seeds, pathogens, and metal toxicities. Composting bestows a tactic for coping high volumes of organic wastes in environmentally sound and desirable manners. Composted materials are remarkably regarded for their ability to improve soil health and plant growth, and suppress pathogens and plant diseases. Currently several composting systems have become available; ranging from a crude and slow windrows method, to the most speedy and computer monitored in-vessel system. Scientific investigations of this biological cum chemical process have reached to molecular level. Value addition of compost through beneficial microorganisms, mineral materials and fertilisers is also being considered. The nature and composition of materials put into composting is imperative for its quality rationale. On the whole, principles and processes governing composting are not so straightforward that ordinary enterprises could develop efficient composting facilities for the treatment of organic wastes. In this scenario, accessibility of comprehensive information to the scientific community as well as environmental protection agencies is imperative. This review article brings together the current information necessary for effective composting of organic wastes from different origins with diversified characteristics under various situations. It also covers the schematic description of well known composting systems, and various factors controlling the process.
Because of its prolific growth, oilseed rape (Brassica napus L.) can be grown advantageously for phytoremediation of the lands contaminated by industrial wastes. Therefore, toxic effect of cadmium on the germination of oilseed rape, the capability of plants for cadmium phytoextraction, and the effect of exogenous application of plant growth regulators to mitigate phytotoxicity of cadmium were investigated. For the lab study of seedlings at early stage, seeds were grown on filter papers soaked in different solutions of Cd 2? (0, 10, 50, 100, 200 and 400 lM). In greenhouse study, seedlings were grown in soil for 8 weeks, transferred to hydroponic pots for another 6 weeks growth, and then treated with plant growth regulators and cadmium. Four plant growth regulators viz. jasmonic acid (12.5 lM), abscisic acid (10 lM), gibberellin (50 lM) and salicylic acid (50 lM); and three levels of Cd 2? (0, 50 and 100 lM) were applied. Data indicated that lower concentration of Cd 2? (10 lM) promoted the root growth, whereas the severe stresses (200 or 400 lM) had negative effect on the establishment of germinating seedlings. Plants treated with any of the tested plant growth regulators alleviated cadmium toxicity symptoms, which were reflected by more fresh weight, less malondialdehyde concentration in leaves and lower antioxidant enzyme activities. The application of abscisic acid to the plants cultivated in the medium containing 100 lM Cd 2? resulted in significantly lower plant internal cadmium accumulation.
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