Effect of ionizing radiation induced by coblet-60 (Co-60) on Escherichia coli cells was investigated. A cellular survival curve and mutant frequencies versus absorbed doses were determined. The results showed that the level of bacteria in survival curve decrease to (2) Log with gamma ray doses increase and D-value was equals (0.079) Gy. After irradiation the mutant frequencies increased with doses increase, the greatest mutant frequency was 3.4 10 -12 (1 mutation for 0.29 10 12 CFU for 150 g of rifampicin) induced by(1) Gy, while the smaller was 0.39 10 -12 (1 mutation for 3.41 10 12 CFU for 150 g of rifampicin) induced by(0.1) Gy.
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