Aim and objective: To investigate the potential relationship between seminal complements (C3 and C4) and immunoglobulin (IgA and IgG) levels and seminal parameters (SPs) in chronic hemodialysis patients. Materials and methods: A cross-sectional study was conducted among patients aged between 18 years and 60 years. The sample comprises 60 males undergoing hemodialysis for >6 months, and 15 healthy males without clinical or laboratory signs of genitourinary and eugonadic tract infections. Spermograms and leukocytopermia, serum hormonal profiles, and seminal measurements of complement fractions (C3, C4) and immunoglobulins (IgA, IgG) were performed. Results: The hemodialysis and healthy groups were similar in age (49.47 ± 05.55 and 50.53 ± 04.24 years, p = 0.06). Average levels of seminal C fractions (C3, C4) were similar between the hemodialysis and healthy groups, between normozoospermia and oligozoospermia hemodialysis subgroups, and in the oligozoospermia hemodialysis subgroup. Average seminal levels of Igs (A and G) differed significantly between the hemodialysis and healthy groups (351.60 ± 035.80 vs 247.40 ± 39.00 mg/L), and in normozoospermia and oligozoospermia hemodialysis subgroups (361.20 ± 36.30 vs 340.60 ± 32.50 mg/L). However, they were similar (p > 0.05) between subgroups of oligozoospermia. The seminal fractions of complete (C3 and C4) and Ig (A and G) did not correlate (p > 0.05) with SPs. All participants had hormonal profiles within the normal range. Conclusion:The complement fractions (C3 and C4) and immunoglobulins (IgA and IgG) showed no relationship with SPs in chronic hemodialysis patients.