O controle de qualidade da água é uma das ações fundamentais para a prevenção de doenças. Uma das causas mais frequentes de morte no mundo são as doenças transmitidas pela água, onde as bactérias contaminantes mais encontradas são Acinetobacter spp, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa e Staphylococcus aureus. A falta da potabilidade da água nem sempre é perceptível à visão ou olfato, sendo necessária uma análise laboratorial para detectá-la. Como a qualidade da água é um fator imprescindível à manutenção da saúde humana, em ambientes de saúde, faz-se necessária a confirmação da qualidade da água oferecida aos pacientes. Assim, foi selecionado o ponto de coleta de amostras de água em uma Unidade de Saúde localizados no município de Itajubá, Minas Gerais. Foi realizada a análise de presença de coliformes totais e de Escherichia coli por utilizando o reagente Colilert®, que é aprovado pelo método padrão para análise de água e esgoto Standard Methods for the Examination of Water and Wastewater. Os resultados foram avaliados por alteração de cor indicativa de presença ou ausência de coliformes totais e E. coli. Na Unidade de Saúde, todas as amostras, foram negativas para coliformes totais e presença de E.coli, indicando que as amostras são próprias para consumo. O objetivo desse trabalho foi verificar a qualidade microbiológica da água que é fornecida a uma Unidade de Saúde e alertar para a importância do monitoramento da qualidade visando a prevenção e disseminação de doenças que podem agravar ainda mais o quadro de pacientes já debilitados.
One of the major health security challenges of the 21st century is the occurrence of microbial infections and bacterial complications that could affect 10 million people by 2050. On the biomaterial field, implant metallic currently replaces partial or total body parts and can fail to be integrated into the body due to infections. This study performs two combined surface modifications on Ti-30Ta alloy, in order to obtain an infection-resistance and osseointegration surface on metallic implants to be tested within bacterial biofilm. The Group 1 investigated surface modifications by the anodization process in the electrolyte glycerol + NH 4 F 0.25% at 30V-9 hours and annealed in 530°C (5°C/min). The Group 2 underwent the same process as Group 1 and, additionally, the samples were immersed in 0.3 M CaCl 2 and 0.5 M Na 2 HPO 4 solutions for hydroxyapatite growth. The substrate was characterized using scanning electron microscopy (SEM), X-ray diffractometer (XRD) and dynamic contact angle. S. epidermidis bacterial adhesion and biofilm formation. The results indicated that the Group 1 shows a higher antimicrobial activity, hydrophilic behavior and potential to be used for metallic implant applications. The Group 2 with the hydroxyapatite film coating did not have an improvement in the antimicrobial response.
The development of devices to controlled the release of drugs are in constant technological innovation. The aim is to improve the release of drugs on target areas. Poly (ε-caprolactone) (PCL) has been widely investigated because of degradation rate, biocompatibility, availability, no toxicity, cost and good adhesion to a large number of drugs. Thus, in the present study was associated polymer PCL with antibiotics tetracycline as local delivery system. PCL was obtained by ring-opening polymerization of monomer Ɛ-caprolactone (Ɛ-CL). The samples were characterized by fourier transformed infrared (FTIR), differential scanning calorimetric (DSC), thermogravimetric analyses (TGA) and X-ray diffraction analysis (X-rays). Likewise, was investigated antimicrobial activity against gram-positive bacteria (S. aureus) and gram-negative bacteria (E. coli, P. mirabilis, P. aeruginosa and K. pneumoniae). According to the results, the antibiotics tetracycline has been successfully incorporated to PCL matrices. They release tetracycline in the ideal rates and shows antibacterial activity. So, this material has a potential to been used in implants for drug release.
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