High hydrostatic pressure treatment not only inactivates the pathogenic and spoilage microorganisms present in the food but also preserves its sensory and organoleptic characters and nutrient value. In this study, the high-pressure destruction kinetics of an important food-borne pathogen Listeria monocytogenes ATCC 19115 was studied in Indian white prawn (Fenneropenaeus indicus) muscle. Headless muscle of prawn was artificially spiked with L. monocytogenes ATCC 19115 at the level of approximately 10 7 CFU/g. The spiked samples were vacuum-packed in an ethylene vinyl alcohol (EVOH) pouch and then subjected to high-pressure treatment at different pressure levels (250, 300, 350 and 400 MPa) for different durations of time. Pressure D values were estimated at different pressure levels. The pressure D values at 250, 300, 350 and 400 MPa were estimated as 34.521, 11.806, 5.92 and 5.099 min, respectively. It was observed that, even after 400 MPa pressure treatment for 12 min, the concentration of the L. monocytogenes population was found to be 3.387 Log 10 CFU/g. The combination of high pressure and potassium sorbate dip treatment (in acidic pH) was found to be very useful in inactivating L. monocytogenes. Potassium sorbate (0.1 %) dip for 15 min coupled with 250 and 350 MPa pressure reduced the level of L. monocytogenes by 2.345 and 5.908 Log 10 CFU/g, respectively. Thus, it can be concluded that pressure treatment alone is not sufficient enough to inactivate this pathogen in prawn muscle. The combination of potassium sorbate with high-pressure treatment is a promising approach to inactivate L. monocytogenes in Indian white prawn muscle.
This paper reports the first documented record of bathyal fish Glyptophidium oceanium Smith & Radcliffe, 1913 from the Indian Ocean. The species is redescribed and meristics and morphometrics were compared with its holotype. Glyptophidium oceanium is very similar to the closely related species G. japonicum Kamohara, 1936 in terms of the number of pseudobranchial filaments and pectoral fin rays, but differs from the latter in having more developed gill rakers, a larger orbit and a more slender body. The report of this poorly known bathyal fish species helps in unravelling the rich deep-sea ichthyofaunal biodiversity of India, which is still not fully investigated.
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