Intellectual and scientific content of the study, surgical procedures, critical revision. ABSTRACT PURPOSE:To evaluate the potential of heterologous platelet-rich plasma (PRP) gel for surgical skin wound healing in rabbits METHODS: Blood from a single healthy dog was used for PRP production, with calcium gluconate added to the PRP to form the gel.Two surgical excisions, one to the right and the other to the left of the dorsal midline, were made in six rabbits. One side was randomly allocated to topical application of a physiological solution, and the other was allocated to treatment with heterologous PRP gel. Clinical assessments (weight, pain sensitivity, coloring, edema, hyperemia, exudation, crust, and granulation) and morphometric evaluations were performed 0, 3, 7, 10, 14, and 17 days postoperatively. Histological analysis was performed on the 17 th day. RESULTS:With the exception of the presence of a crust at day 10, clinical variables did not differ significantly between the experimental groups. In both the control and PRP-treated groups, differences were identified when comparing time-points in terms of wound area reduction. Histological results indicated no significant differences between the control group and the PRP-treated group. CONCLUSION:Heterologous platelet-rich plasma gel promoted dermal wound healing in rabbits with no adverse effects.

The use ofdrugs of abuseis apublic health problem. One of thedifficulties intreatingdrug usersis thelack of informationon the subjectfacingdentistry.This study aimed toassessthe oral conditionsofaddicts in treatment in Centersfor Psychosocial Care (CAPS IIAD). We performedan interview, examinationof the oral cavity, sialometryassessmentandoralpHin 60patients attendingtwo CAPS IIADin twocitiesin the stateof SãoPaulo, of both sexesandover 18 years old. The majority(28%) were agedbetween 41and 50 years, were male(85%) andhave elementary education(37%). Among those interviewed,98%reported that they hadbeen to the dentist. 22% did notbrush their teethand 95% did not flossing. The abuse drugsmore used were: alcohol (95%), cigarettes (85%), marijuana(48%) and cocaine/ crack/merla(40%). The oral routeisthe route of administrationof the drugmost frequently cited(51%). Hyposalivationwas diagnosedin 56% of patients and 12% hadacidic pH. The main visible changesin periodontalarea weregingival retraction, color change, plaque and calculussimultaneously (42%), anddental change wasexogenous pigmentation(15%). Only 2% had none decayed, restored,lostorextraction indicated in permanent teeth. All patients had multipleoral abnormalitiesassociated with use ofdrugs of abuse(such ashyposalivation, oralacidpH, exogenous pigmentation), and oral healthgreatlycompromised.

-Background -Helicobacter pylori is the most prevalent infectious agent worldwide. About 90% of patients with chronic gastritis are infected with this bacterium. Some studies have shown a association between the H. pylori concentration and the scores of gastritis activity and severity. Aim -To evaluate the association between H. pylori concentration and the combining frequency of findings on histopathological examination. Methods -Two hundred consecutive gastric endoscopic biopsies diagnosed as chronic gastritis were retrospectively investigated. The Warthin-Starry silver stain was used to study H. pylori and the following parameters were assessed (according to the Sydney system): 1. infiltration by polymorphonuclear cells in chorio and epithelium (activity) graded as mild, moderate and marked; 2. presence of lymphoid follicles; 3. presence of intestinal metaplasia; 4. presence of regenerative cell atypias, graded as mild, moderate and marked; and 5. H. pylori concentration on the mucous covering the foveolar epithelium. Results -The most frequent association was chronic gastritis and activity, regardless of H. pylori concentration. The association of five histopathological findings in the same biopsy has not occurred in the cases of higher concentration of H. pylori. Conclusion -Our study has not revealed any association between H. pylori concentration and an increasing in the number of histopathological findings found in the gastric mucosa. Since referring to its presence is much more important than to its concentration. HEADINGS -Gastritis. Helicobacter pylori. Helicobacter infections. Biopsy.

Objective: This study aims to evaluate the effect of the pH of drinking water in the oral changes caused by cadmium poisoning. Material and method: Ninety male Wister rats were divided into the following six groups: A - 15 rats were given cadmium chloride solution (400 mg/L) in drinking water with a neutral pH (pH 7.0); B - 15 rats received cadmium chloride solution (400 mg/L) in drinking water with an acidic pH (pH 5.0); C - 15 rats were treated with a cadmium chloride solution (400 mg/L) in drinking water with a basic pH (pH 8.0); D - 15 rats received drinking water with an acidic pH (pH 5.0); E - 15 rats were given drinking water with a basic pH (pH 8.0); F - 15 rats received water with a neutral pH (pH 7.0). All animals were sacrificed six months after the beginning of the experiment. A biopsy of the buccal mucosa, tongue and salivary gland of each animal was taken for microscopic analysis. Result: No changes were observed in the buccal mucosa, tongue mucosa or salivary glands in any of the groups. Conclusion: Drinking water that contains a high concentration of cadmium with differing pH levels demonstrated no damage to the oral mucosa and salivary glands of male Wistar rats.

Objectives The present study aimed to assess the antimicrobial and antiadhesion behavior of quercetin‐loaded chitosan nanoparticles in Escherichia coli and Staphylococcus aureus multidrug‐resistant isolates. Methods The ionic gelation method was used to prepare chitosan nanoparticles loaded with quercetin. The antimicrobial and antibiofilm effects were observed by minimum inhibitory concentration (MIC), plate count, crystal violet assay, and the matrix exopolysaccharide dosages. The nanoparticles coated in silicone urethral catheters were evaluated by crystal violet assay and plating count method. Results MIC ranged from 6.25 to 12.5 mg/ml. A reduction of at least 3.6 log CFU/ml and 6.2 log CFU/ml for, respectively, E. coli and S. aureus isolates was observed (p < 0.05). Under subinhibitory concentration (3.1 mg/ml) it was found a reduction of microbial adhesion and exopolysaccharide dosages in respectively 83.3% and 75% of the bacterial samples. The coated silicone urethral catheters showed a reduction of adhered cells in 25% of the isolates and biomass decreasing in 91.6% of them (p < 0.05). Conclusions The quercetin nanoparticles provided antimicrobial and antiadhesion effects in multidrug‐resistant isolates.