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The behavior of human immunoglobulin G (IgG) and antigen-binding fragment (Fab fragment) adsorption onto phospho-L-tyrosine immobilized on agarose (P-Tyr-agarose) was evaluated by pseudoaffinity chromatography. The effects of buffer systems MES, MOPS, Bis-Tris, Tris-HCl and sodium phosphate (NaP) and pH on IgG adsorption were studied and high purity values were obtained (96%, based on ELISA analysis of albumin, transferrin and immunoglobulins A, G and M) when IgG was purified from human plasma diluted in 10 mmol L −1 NaP buffer at pH 6.0. The capture of IgG by the P-Tyragarose was also promising, since 91% of the IgG was adsorbed when plasma was diluted in 25 mmol L −1 MES buffer at pH 5.5, recommending its use for IgG depletion from human plasma under this condition. The experimental data on IgG adsorption kinetics were in agreement with the pseudo-second-order model. The adsorption isotherm data were well described by the Langmuir-Freundlich model with the value of parameter n being <1 (0.72), indicating negative cooperativity. Selectivity was achieved on P-Tyr-agarose from digested human IgG in HEPES 25 mmol L −1 buffer at pH 7.0where Fab fragments were obtained in eluted fractions without Fc fragments (but with uncleaved IgG) with 86.2% recovery.

Nanoparticle, SEM and TEM.The recent development and implementation of new technologies has led to new era of nano revolution which unfolds the role of plants in bio and green synthesis of nano materials [1].Metallic nanoparticles are being utilized in every phase of science along including medical fields and are still charming the scientists to explore new dimensions, generally attributed to their corresponding small sizes. The upcoming researches have proven their antimicrobial significance [2]. Although nanoparticles can be synthesized by an array of conventional methods, green route of synthesis is competent over physical and chemical methods [3]. In the present work nickel nanoparticles was synthesized using clove and extracts. The absorption maxima nickel is at 680nm. The morphology of the particles was confirmed by transmission microscopy (TEM) and Scanning electron microscopy (SEM). TEM results suggest the size of the particle is in the range of 50 -200nm. SEM images show the particles are spherical and granular nature. The synthesized nanoparticles s how fairly good anti microbial activity.Copy Right, IJAR, 2016,. All rights reserved. …………………………………………………………………………………………………….... Introduction:-Nanobiotechnology is an enabling technology that deals with nanometer-sized materials in diverse fields of science such as biotechnology, nanotechnology, physics, chemistry and materials science. In addition to many physical and chemical methods which have been developed for preparing metallic nanoparticles, nanobiotechnology also serves as a significant technique in the progress of clean, non-toxic and environment friendly procedures for synthesis and assembly of metallic nanoparticles. The biosynthesis of nanoparticles has attracted attention of many researchers owing to their physical and chemical processes being expensive and drastic reaction consitions.Nickel nanoparticles, due to their excellent physical and chemical properties and low cost of preparation have been of great interest. Copper nanoparticles havewide applications as heat transfer systems,antimicrobial materials, super strong materials, sensors and catalysts Nickel nanoparticles are very reactive because of with other particles [1] and increase their antimicrobial efficiency. Colloidal Nickel has been used as an antimicrobial agent for decades. Nickel nanoparticles (2-5 nm) have revealed a strong antibacterial activity and were able to decrease the microorganism concentration by 99.9%. Due to the stability of Nickel nanoparticles supported on a matrix and their disinfecting properties.

RESUMO -A captura de IgG por cromatografia utilizando aminoácidos e seus derivados como ligantes tem sido realizada com sucesso e em muito devido à diferentes interações (hidrofóbica, eletrostática, ligação π-π, etc) entre a proteína alvo e o ligante. A combinação de duas ou mais interações caracteriza a cromatografia de interações mistas (MMC), que tem proporcionado bons resultados na adsorção de IgG. Este trabalho visa o estudo da adsorção de IgG a partir do plasma humano em orto-fosfo-L-tirosina (OPT) imobilizado em agarose. Os resultados demonstraram que a interação entre IgG e o OPT é predominantemente eletrostática. Observou-se que o aumento do valor de pH diminuiu a capacidade de adsorção, no entanto, observou-se maior seletividade na adsorção de IgG. Homogeneidade eletroforética foi observada para os tampões Bis-Tris e Mops em pH 7,0 e 7,5, respectivamente.Área temática: Processos Biotecnológicos 1