SummaryPlants are subject to attack by a wide range of phytopathogens. Current pathogen detection methods and technologies are largely constrained to those occurring post-symptomatically. Recent efforts were made to generate plant sentinels (phytosensors) that can be used for sensing and reporting pathogen contamination in crops. Engineered phytosensors indicating the presence of plant pathogens as early-warning sentinels potentially have tremendous utility as wide-area detectors. We previously showed that synthetic promoters containing pathogen and/ or defence signalling inducible cis-acting regulatory elements (RE) fused to a fluorescent protein (FP) reporter could detect phytopathogenic bacteria in a transient phytosensing system. Here, we further advanced this phytosensing system by developing stable transgenic tobacco and Arabidopsis plants containing candidate constructs. The inducibility of each synthetic promoter was examined in response to biotic (bacterial pathogens) or chemical (plant signal molecules salicylic acid, ethylene and methyl jasmonate) treatments using stably transgenic plants. The treated plants were visualized using epifluorescence microscopy and quantified using spectrofluorometry for FP synthesis upon induction. Time-course analyses of FP synthesis showed that both transgenic tobacco and Arabidopsis plants were capable to respond in predictable ways to pathogen and chemical treatments. These results provide insights into the potential applications of transgenic plants as phytosensors and the implementation of emerging technologies for monitoring plant disease outbreaks in agricultural fields.

A survey was conducted of nursery operators participating in workshops in west-central Florida. The purpose of the survey was to identify the irrigation best management practices (BMPs) adopted by container nurseries in west-central Florida and obtain information regarding emphasis of future extension educational programs. Workshops were conducted in Hillsborough County, Fla., and Manatee County, Fla., and participation was voluntary. Respondents were asked about BMPs used in the nurseries according to the irrigation system used and it was found that the majority of the nurseries relied on well water as the primary source for irrigation. While 69% of the nurseries monitored uniformity of microirrigation systems, only 35% monitored uniformity of overhead irrigation systems. Thirty-four percent of the nurseries collected irrigation or rain runoff and 9% knew the water holding capacity of their substrate. Most of the nurseries grouped plants by irrigation requirements (74%) and grouped container sizes by irrigation requirements (69%). The survey indicates that many BMPs are not widely adopted by nurseries in west-central Florida. The information from this survey can be used as a guide to focus the efforts of university extension educational programs to achieve greater adoption of BMPs.

A micropropagation protocol for shoot culture of sweet viburnum (Viburnum odoratissimum) is described. Nodal explants, initially established on MS medium, were transferred to WPM supplemented with combinations of BA and GA 3 . Maximum shoot multiplication was observed on explants cultured on medium supplemented with BA concentration higher than 1.1 lM, and 14 lM GA 3 . Although Stage II medium supplemented with BA concentration higher than 1.1 lM resulted in increased shoot multiplication, it also caused a decrease in shoot length. A negative carry over effect of GA 3 on rooting was observed in subsequent Stage III cultures. The presence of GA 3 in Stage II medium promoted shoot elongation, but it also caused a decrease in microcutting rooting. For this reason, 0.5 lM BA and 14 lM GA 3 were selected for optimum Stage II shoot multiplication. Although 100% microcuttings formed roots when cultured on medium containing 6.0 lM NAA, significant callus formation was observed and ex vitro survival rate was low (49%). Rooting was achieved after 3 weeks with 82% of microcuttings on medium supplemented with 3 lM IBA. The survival rate of plantlets under ex vitro conditions was 100% after 3 weeks. Plants looked healthy with no visually detectable phenotypic variation based on observation of about 30 plants.

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Most trees and shrubs have cyclic, rather than continuous shoot and root growth. Growth cycles have important implications with regards to transplant and fertilization timing. Although growth cycles of several woody species have been characterized, no information is available on sweet viburnum (Viburnum odoratissimum). A series of experiments was conducted to study shoot and root growth flushes of sweet viburnum and the influence of nitrogen fertilization on flushes. Plants were grown in observation tubes and kept under greenhouse conditions. Sweet viburnum exhibited alternating periods of root and shoot elongation, and the root elongation peak preceded shoot elongation peak by 6-18 days. Increased nitrogen fertilization rate negatively impacted the magnitude and number of root growth flushes. Further research is needed to determine when maximum nitrogen uptake is occurring, relative to root and shoot growth cycles.