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The fragmentation of forests is a consequence of human activities that intensively change the natural landscapes. In Brazil, there is a long-standing legal framework for native vegetation protection, called Forest Code. The last update is from 2012. The revisions incorporated in this restructured code (Law No. 12,651/12) encompassed new rules, rights and obligations for adequate land use management of productive systems and for environmental conservation and protection. An example of a protective measure was the creation of a Legal Reserve inside the rural properties and Areas of Permanent Protection around the watercourses. This study explored the effects of implementing the new code on the spatial and temporal evolution of forest cover fragmentation since the legal changes were set up. In that context, the area, perimeter and shape (circularity index) of forest fragments, as well as the buffer strips along watercourses, were assessed from 2010 to 2016 within the municipality of Ribeirão Preto, São Paulo state, Brazil. The assessment resorted to remote sensing techniques and visual inspection of orbital images. It was clear from the results that forest patches became more abundant but smaller, more dispersed and elongated, and that this route to fragmentation was hinged on a chaotic expansion of urban and agricultural areas. Important edge effects were anticipated from these results. The area numbers revealed that Ribeirão Preto was 7.95% covered with forest fragments in 2010, while this percentage rose to 8.03% in 2013 and reached 8.35% in 2016. Most of this increase occurred within a 30-meter wide buffer along the watercourses. This was a positive outcome. However, the numbers also revealed that forested areas smaller than 51 hectares increased from 71% in 2010 to 73% in 2016. The conclusions were that implementation of Law No. 12,651/12 is leading to an increase of forestland, but not yet promoting sustainability, namely through improved connectivity among fragments for reduction of edge effects and provision ecosystem services.

Water samples, zooplankton community and sediment samples were analyzed at three sites in a pond receiving wastes, bi-weekly for seven months, covering the rainy and dry seasons. Water quality parameters failed to show significant differences between the sites for turbidity, total suspended solids, dissolved oxygen, transparency, conductivity, alkalinity and total phosphorous. Only temperature was different between the seasons. In the case of sediment, only aluminum (Al) was not different during the experimental period when highest calcium (Ca) concentrations were reported. The relative abundance of Rotifera during the sampling period reached 80-96% (rainy season) and 59-98% (dry season) in total zooplankton. Current study demonstrated that the water quality of shallow pond associated with allochthonous materials from aquaculture farm significantly influenced the structure of zooplankton assemblage due to high nutrient concentrations, conductivity, alkalinity and chlorophyll-a causing more fertilized water. However, heavy rains (February-April) and continuous water flow favored aeration (dissolved oxygen >5.2 ± 1 mg L-1). The use of pond water for agricultural irrigation or for other purposes, must be analyzed more effectively, avoiding problems caused by its usage.

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