Three species of Catfishes (Mystus vitattus, Clarius batrachus and Heteropneustus fossilis) were analyzed for proximate composition i.e. Moisture and Protein content to evaluate their nutritional value. The Moisture content of the fish species ranged between 71.2 ± 0.07% to 79.45±0.04%. The muscle protein content of the fishes were ranged between 14.49±0.96% to 18.14±0.07%.The mean values of moisture and Protein content were found statistically significant (P<0.05) among all three species. The moisture content in Mystus vittatus was higher than the other samples at highly significant (p<0.001) level. The amount of moisture content in Clarius batrachus is higher than Heteropneustus fossilis but lower than Mystus vittatus at highly significant level (p<0.001). There was a non significant (p>0.05) difference between moisture content in Mystus vittatus and Clarius batrachus. The Protein content in Heteropneustus fossilis is higher than that of the other fish samples at highly significant (p<0.001) level. The protein content in Clarius batrachus is higher than Mystus vittatus but lower than Heteropneustus fossilis at highly significant level (p<0.001). The protein content in Mystus vittatus and Clarius batrachus is none significantly (p>0.05) different. Variation of protein content of different fishes helps nutritionists and researchers who are striving to improve the nutritive value, Processing and marketing of those fish species and in fishing industry. The experiment were conducted during a time period of June 2019 to February 2020. Fish samples were collected from Tihu local market and were brought to the