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During the last 20 years, the reefer shipping sector has been characterised by a gradual changing process, leading from specialised vessels to reefer containers. In fact, the reefer bulk fleet has experienced an irreversible decline, while the reefer container fleet has shown a continuous growth. This article investigates the current major factors shaping the reefer shipping industry, in order to understand the economic drivers inducing competition between bulk and containers. Therefore, a twofold analysis has been performed. First, an overview of the reefer market, including the cold chain, the demand for refrigerated products and the supply of reefer capacity given both by containership fleet and by conventional reefer fleet. Second, a specific case of the banana trade has been carried out for evaluating the revenues of each player involved in the banana cold chain followed by a cost analysis. The results reveal the market tendencies, focusing on the main sources of competition for bulk versus container.

One of the mechanisms by which viruses can evade the host’s immune system is to modify the host’s DNA methylation pattern. This work aims to investigate the DNA methylation and gene expression profile of COVID-19 patients, divided into symptomatic and asymptomatic, and healthy controls, focusing on genes involved in the immune response. In this study, changes in the methylome of COVID-19 patients’ upper airways cells, the first barrier against respiratory infections and the first cells presenting viral antigens, are shown for the first time. Our results showed alterations in the methylation pattern of genes encoding proteins implicated in the response against pathogens, in particular the HLA-C gene, also important for the T-cell mediated memory response. HLA-C expression significantly decreases in COVID-19 patients, especially in those with a more severe prognosis and without other possibly confounding co-morbidities. Moreover, our bionformatic analysis revealed that the identified methylation alteration overlaps with enhancers regulating HLA-C expression, suggesting an additional mechanism exploited by SARS-CoV-2 to inhibit this fundamental player in the host’s immune response. HLA-C could therefore represent both a prognostic marker and an excellent therapeutic target, also suggesting a preventive intervention that conjugate a virus-specific antigenic stimulation with an adjuvant increasing the T-cell mediated memory response.

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