To cite this article: Francavilla R, Calasso M, Calace L, Siragusa S, Ndagijimana M, Vernocchi P, Brunetti L, Mancino G, Tedeschi G, Guerzoni E, Indrio F, Laghi L, Miniello VL, Gobbetti M, De Angelis M. Effect of lactose on gut microbiota and metabolome of infants with cow’s milk allergy. Pediatric Allergy Immunology 2012: 23: 420–427. Abstract Allergic infants have an unusual gastrointestinal microbiota with low numbers of Bifidobacterium/Lactobacilli and high levels of Clostridium, staphylococci and Escherichia coli. Hydrolyzed formula used to treat these infants is deprived of lactose that instead may influence the gut microbial composition. The aim of the present study is to investigate the influence of lactose on the composition of the gut microbiota and metabolome of infants with cow’s milk allergy. Infants prospectively enrolled received an extensively hydrolyzed formula with no lactose for 2 months followed by an identical lactose‐containing formula for an additional 2 months. Healthy, age‐gender‐matched infants were used as controls. The following determinations were performed before and after the introduction of lactose in the diet: enumeration of cells present in the feces using FISH, counts of viable bacterial cells and gas‐chromatography mass spectrometry/solid‐phase microextraction analysis. The addition of lactose to the diet significantly increases the counts of Bifidobacteria and lactic acid bacteria (p < 0.01), decreases that of Bacteroides/clostridia (p < 0.05) reaching counts found in healthy controls; lactose significantly increases the concentration of total short‐chain fatty acids (p < 0.05). The addition of lactose to an extensively hydrolyzed formula is able to positively modulate the composition of gut microbiota by increasing the total fecal counts of Lactobacillus/Bifidobacteria and decreasing that of Bacteroides/Clostridia. The positive effect is completed by the increase of median concentration of short chain fatty acids, especially for acetic and butyric acids demonstrated by the metabolomic analysis.

Abstract:Land use affects eco-hydrological processes with consequences for floods and droughts. Changes in land use affect ecosystems and hydrological services. The objective of this study is the analysis of hydrological services through the quantification of water resources, pollutant loads, land retention capacity and soil erosion. On the basis of a quantitative evaluation, the economic values of the ecosystem services are estimated. By assigning an economic value to the natural resources and to the hydraulic system, the hydrological services can be computed at the scale of catchment ecosystem. The proposed methodology was applied to the basin "Bonis" (Calabria Region, Italy). The study analyses four land use scenarios: (i) forest cover with good vegetative status (baseline scenario); (ii) modification of the forest canopy; (iii) variation in forest and cultivated surfaces; (iv) insertion of impermeable areas. The simulations prove that the variations of the state of forest areas has considerable influence on the water balance, and then on the provided economic value. Small economic changes derive from reducing the impermeable areas. Increasing the agricultural area to 50% of the total, and reducing the forest surface, affects soil erosion, reduces the storage capacity of the water, and consequently the water harvesting. The suggested methodology can be considered a suitable tool for land planning.

The Pressure Chamber, the most popular method used to measure xylem water potential, is a discontinuous and destructive technique and therefore not suitable for automated monitoring. Continuous non-destructive monitoring could only be achieved until very recently via the Thermocouple Psychrometer (TP). We here present the High-Capacity Tensiometer (HCT) as alternative method for continuous non-destructive monitoring. This provided us with a unique chance to cross-validate the two instruments by installing them simultaneously on the same sapling stem. The HCT and the TP showed excellent agreement for xylem water potential &lt; -0.5 MPa. Response to day/night cycles and watering was remarkably in phase, indicating excellent response time of both instruments despite substantially different working principles. For xylem water potential &gt; -0.5 MPa, the discrepancies sometimes observed between the HCT and TP were mainly attributed to the kaolin paste used to establish contact between the xylem and the HCT, which becomes hydraulically poorly conductive in this range of water potential once dried beyond its air-entry value and subsequently re-wetted. Notwithstanding this limitation, which can be overcome by selecting a clay paste with higher air-entry value, the HCT has been shown to represent a valid alternative to the TP.

The response of the shallow portion of the ground (vadose zone) and of earth structures is affected by the interaction with the atmosphere. Rainwater infiltration and evapotranspiration affect the stability of man-made and natural slopes and cause shallow foundations and embankments to settle and heave. Very frequently, the ground surface is covered by vegetation and, as a result, transpiration plays a major role in ground-atmosphere interaction. The soil, the plant, and the atmosphere form a continuous hydraulic system, which is referred to as Soil-Plant-Atmosphere Continuum (SPAC). The SPAC actually represents the ‘boundary condition’ of the geotechnical water flow problem. Water flow in soil and plant takes place because of gradients in hydraulic head triggered by the negative water pressure (water tension) generated in the leaf stomata. To study the response of the SPAC, (negative) water pressure needs to be measured not only in the soil but also in the plant. The paper presents a novel technique to measure the xylem water pressure based on the use of the High-Capacity Tensiometer (HCT), which is benchmarked against conventional techniques for xylem water pressure measurements, i.e. the Pressure Chamber (PC) and the Thermocouple Psychrometer (TP).

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