Viral contamination in cell culture media is an important issue in the biopharmaceutical manufacturing industry and may have serious consequences on product quality, efficacy, and safety. High-temperature/short-time (HTST) treatment of cell culture media is one of the proven techniques used in the industry for the prevention and mitigation of media viral contamination. With the HTST method, the formulated media is pasteurized (virus-deactivated) by heating at preset conditions. This paper provides the identification and root-cause study of the media precipitates that adversely affected the HTST process and discusses the possible solutions to mitigate the precipitation problem.

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Ozone dissolution system design is important for meeting transfer efficiency (TE) goals. Large sidestream pump flow (L) and high venturi inlet pressure improves TE but increases operating cost. Ozone TE was examined at a 25 gpm (97-Lpm) pilot-scale sidestream system with (SS w-dg ) and without (SS wo-dg ) degas separation. Under constant ozone dose conditions, process operating parameters were varied including sidestream gas/liquid (G/L) ratio, venturi-inlet water pressure, venturi-outlet water pressure, feed gas pressure, and ozone gas concentration. Performance results included determination of TE, ozone exposure (CT HDT ), and hydraulic detention time (T HDT ). Several design aspects of sidestream ozone systems were examined to improve mass transfer by using remixing devices, protecting ozone gas piping from corrosion, calculating sidestream ozone residual, and driving force for mass transfer. Moisture contamination of ozone supply lines may cause corrosion and/or decomposition of ozone gas that releases heat and destroys ozone. Ozone gas piping design is critical to prevent trapping water that might enter gas pipe during power outage or when units are offline. During plant operation below design flow, multiple constant speed pumps or variable speed pumps were evaluated to reduce overall operating costs. ARTICLE HISTORY

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