SummaryTo identify patterns in the temporal dynamics and spatial organization of kangaroo subpopulations that are commercially harvested, we fitted multivariate autoregressive state‐space (MARSS) models to time series of kangaroo abundance from the main harvest zones of eastern Australia (1990–2019 for New South Wales and 1984–2019 for Queensland). We found that the responses of most populations to process variation, that is variation due to environmental or demographic stochasticity were correlated and that populations responded synchronously to environmental changes. Furthermore, we examined the influence of the covariates lagged rainfall, Southern Oscillation Index (SOI) and harvest rate on kangaroo abundance. We found that lagged rainfall had a positive influence, SOI and harvest rate had negative influences but the influence of covariates was not consistent across species or between subpopulations. In terms of population spatial structure, the analysis identified two subpopulations of Red Kangaroo (Osphranter rufus) in New South Wales and four subpopulations of grey kangaroos (a combination of Eastern Grey Kangaroo (Macropus giganteus) and Western Grey Kangaroo (Macropus fuliginosus)), which appeared to be associated with a rainfall gradient from east‐west. In Queensland, separate subpopulations were identified in each of the three main harvest management zones for both Red Kangaroo and Eastern Grey Kangaroo. The implications of these results for the management of commercially harvested kangaroos are discussed.

Background The SARS-CoV-2 pandemic necessitated rapid and global responses across all areas of healthcare, including an unprecedented interest in serological immunoassays to detect antibodies to the virus. The dynamics of the immune response to SARS-CoV-2 is still not well understood. Methods We measure SARS-CoV-2 antibody levels in plasma samples from 880 people in Northern Ireland by Roche Elecsys Anti-SARS-CoV-2 IgG/IgA/IgM, Abbott SARS-CoV-2 IgG and EuroImmun IgG SARS-CoV-2 ELISA immunoassays to analyse immune dynamics over time. Using these results, we develop a "pseudo gold standard" reference cohort against which to assess immunoassay performance. We report performance metrics for the UK-RTC AbC-19 rapid lateral flow immunoassay (LFIA) against a characterised panel of 304 positives established using the "pseudo gold standard" system and 350 negative samples. Results We detect persistence of SARS-CoV-2 IgG up to 140 days (20 weeks) post infection, across all three antibody immunoassays, at levels up to 4.4 times the cut-off for a positive result by Roche measurement. Using our "pseudo gold standard" cohort (n=348 positive, n=510 negative) we determine the sensitivity and specificity of the three commercial immunoassays used (EuroImmun; Sens. 98.9% [97.7-99.7%]; Spec. 99.2% [98.4-99.8%]; Roche; Sens. 99.4% [98.6-100%]; Spec. (96.7% [95.1-98.2%]; Abbott; Sens. 86.8% [83.1-90.2%]; Spec. (99.2% [98.4-99.8%]). The UK-RTC AbC-19 lateral flow immunoassay using shows a sensitivity of 97.70% (95.72%-99.34%) and specificity of 100% (100.00-100.00%). Conclusions Through comprehensive analysis of a large cohort of pre-pandemic and pandemic individuals, we show detectable levels of IgG antibodies, lasting up to 140 days, providing insight to immunity levels at later time points. We propose an alternative to RT-PCR positive status as a standard for assessing SARS-CoV-2 antibody assays and show strong performance metrics for the AbC-19 rapid test.

SummaryThis paper describes advances made to aerial surveys of macropods in New South Wales and Queensland. Regular aerial surveys of macropods across large areas of Australia’s arid and semi‐arid rangelands were established as a response to concerns for the species’ conservation. They became integral to the harvest management of the four macropod species – Eastern Grey Kangaroo (Macropus giganteus), Western Grey Kangaroo (M.fuliginosus), Red Kangaroo (Osphranter rufus) and Common Wallaroo (O.robustus). Harvest quotas are set as a proportion of population size, requiring absolute estimates of abundance. Counts were made in strip transects from fixed‐wing aircraft, but required correction for animals that are missed. In New South Wales and Queensland, counts are now made using line transect sampling, providing survey‐specific correction. Helicopters are used in more rugged and timbered areas and where Common Wallaroo can be more frequently found and harvested. Mark‐recapture distance sampling is used in more open country that can be surveyed using fixed‐wing aircraft and improves the accuracy of density estimates by accounting for animals missed on the transect line. Macropod populations are likely to be surveyed in the future by airborne imagery and by drones, but the technology needs further development. Spatial models could usefully provide small‐scale density estimates and address non‐random sampling.