Objectives: The overall goal was to analyze genetic diversity in cultivars of Musa acuminata (Colla) and M. balbisiana (Colla), commonly grown in farms from Caldas department. Scope: Characterization of the genetic variability, at the molecular and morphological level of cultivars of M. acuminata and M. balbisiana, found in farms from Caldas farmers using morphological descriptors and fluorescent microsatellites. Methodology: Phenotyping evaluations comprised 57 morphological characters following the descriptors proposed by IPGRI for the Musa genus, and for genotyping evaluations, nine fluorescent microsatellites (Simple Sequence Repeats-SSR) were used to allow the precise identification of alleles. Additionally, cluster analyses were carried out independently for both morphological and genotypic characterizations under Principal Component Analysis (PCA) and Bootstrapping methods respectively. Main results: Positive and negative highly significant correlations were found for the morphological descriptors, where traits such as presence/ absence of male bud was the rule, as well as the diameter and perimeter of this trait, plus the diameter and perimeter of the peduncle, number of fruits, pseudostem height and fruit length contributed considerably to the variability among the cultivars allowing the discrimination of three main groups in the cluster analyzes. From the molecular perspective a total of 72 polymorphic alleles were obtained, with an average genetic diversity of 0,79, polymorphic information content (PIC) of 0,77 and heterozygosity of 0,48, showed a moderate degree of genetic differentiation (FST = 0,061) among Musa cultivars, generating three main sub-clusters based on their genetic dissimilarity. Conclusions: The identification of certain morphological traits showed to be suitable for the discrimination of Musa cultivars evaluated here. On the other hand, molecular characterization allowed to establish the genetic relationships among groups, also fluorescent SSR were highly informative and accurate, in such a way that can be considered suitable for characterizations in Musa varieties.
The objective of this research was to evaluate genotypes of iron- and zinc-enriched common beans during breeding in producers’ farms. Yield, disease reaction, and commercial grain characteristics were evaluated to achieve this objective. In three locations of Antioquia (Rionegro, Jardín, and Betulia), seven bush beans and eight climbing bean genotypes were planted. A randomized complete block design with four replications was used in each location. There were significant differences between the bush and climbing bean genotypes that were evaluated. The highest yields, in all locations, were for the biofortified bean NUA 45 and the control variety Uribe Rosado, followed by the CAL 96 and AFR 612 genotypes. For the climbing beans, the highest yields were found in the G2333 genotypes, being this treatment equal to the MAC 27, a bean that is adapted to mid-climate and altitudes. The MAC 27 material is presented as a promising variety because of its high yields and tolerance to diseases, mainly anthracnose.
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