Aim: The present study was undertaken to estimate the genetic parameters and to assess the inheritance pattern of egg quality traits in White Leghorns. Materials and Methods: Data on 480 eggs produced by 40 week old pullets of 4 genetic groups were used for studying egg quality traits. Heritabilities and correlations were estimated by full sib correlation method using Mixed Model Least Squares and Maximum Likelihood (LSMLMW) computer program. Results: The egg weight, haugh unit, yolk index, albumen index, yolk weight, albumen weight, shell weight and shell thickness ranged from 50.01 ± 0.48 to 53.89 ± 0.43 g, 65.38 ± 0.92 to 80.98 ± 1.01, 0.341 ± 0.003 to 0.353 ± 0.003, 0.056 ± 0.002 to 0.087 ± 0.002, 14.16 ± 0.13 to 15.58 ± 0.12 g, 30.92 ± 0.39 to 33.18 ± 0.39 g, 4.32 ± 0.05 to 5.12 ± 0.05 g and 0.336 ± 0.003 to 0.376 ± 0.003 mm, respectively. Heritability estimates for egg weight, yolk index, albumen index and albumen weight ranged from low to medium while those of haugh unit, yolk weight, shell weight and shell thickness ranged from low to high. The genetic and phenotypic correlations of egg weight with other egg quality traits except shell quality traits were mostly positive and moderate to high. High positive genetic and phenotypic correlations between haugh unit and other traits were observed. Genetic correlation of yolk weight with albumen weight was positive while that with shell quality traits was mostly negative. Shell weight was positively correlated with shell thickness. Conclusion: Significant genetic group differences were observed for various egg quality traits studied. The heritability estimates for different egg quality traits were low to moderate. The association among egg quality traits was positive in general. [Vet World 2013; 6(5.000): 263-266

Canine adenovirus (CAV) circulates as two distinct serotypes, CAV-1 and CAV-2, which are antigenically related but differ in their clinical manifestations. CAV is one of the important viral agents in the etiology of canine gastroenteritis. Here, we report the molecular surveillance and genetic characterization of CAV from clinical cases of canine gastroenteritis. A total of 302 fecal/rectal swabs were collected from dogs presented with gastroenteritis at various clinics in and around Hyderabad, India during 2018-19. These samples were tested for CAV using polymerase chain reaction with primers designed for the CAV E3 gene and the virus was isolated from positive samples. CAV-2 nucleic acid was present in 4.9% of the test samples. The partial sequence analyses of the E3 gene of the CAV-2 isolates revealed a frameshift mutation by insertion of nucleotide "G" at 1077 position of E3 gene, which resulted in an extension of the polypeptide chain by eleven amino acids. As a result, isolates from the current study formed a novel group, and the virus that was previously subdivided into two groups worldwide is now categorized under three. The study identifies a novel group of CAV-2 circulating in India providing an updated information regarding CAV-2.

Aim : To study the influence of housing system and dietary supplementation of probiotics and enzymes on haematological parameters of rabbits. Materials and Methods: A total of 144 weaned rabbits were divided into 2 groups of 72 in each group and housed under conventional cage system and backyard system. The rabbits in each housing system were divided into 4 groups of 18 in each group and the diets were supplemented with probiotics, enzymes and both. Results: The housing system and supplementation of probiotics and enzymes did not exert significant influence on any of the haematological parameters studied. However, there was slight positive influence of probiotic and enzyme supplementation on the health status of rabbits as revealed by haematological parameters. The overall mean Total erythrocyte count, total leucocyte count, lymphocytes, neutrophils, eosinophils, monocytes, haemoglobin and packed cell volume were 7.52, 6.29 (103/mm3), 60.27%, 35.71%, 1.35%, 1.92%, 10.67 g/dl and 34.25%, respectively. Conclusion: Rabbits can be reared on low input backyard system without any adverse effect on health and supplementation of probiotics and enzymes had a positive influence on health status of rabbits.

Aim:The main intension of this research work is to develop a feeding system for growing Nellore ram lambs by feeding maize silage based rations supplemented with concentrate and or legume hay at certain levels to investigate the growth rate. Materials and Methods:Experimental animals (Nellore ram lambs) were purchased from local sandy and maize silage was prepared at village by using silos (9''L X 9''W X 8''D) and concentrate mixture was prepared at feed mill located at department by using locally available ingredients. Animals were housed in well ventilated sheds and were dewormed and vaccinated against diseases. Fortnightly body weights were taken by using spring balance and calculated the total weight gain and ADG. The experimental animals were divided into seven groups based on their body weight to contain six in each and were fed intensively for five months with sole maize silage (R-I), silage + concentrate at 0.5 per cent body weight (R-II), silage + concentrate at 1.0 per cent body weight (R-III), silage + concentrate at 1.5 per cent body weight (R-IV), silage + lucerne hay (R-V) and silage + GN haulms (R-VI) and sweet sorghum bagasse based complete diet (R-VII) to study the growth rate.Results: Significantly (P<0.01) increase in ADG was observed in ram lambs fed R-IV ration was 16.58, 20.49, 24.10, 29.74, 38.28 and 62.01 per cent, respectively in comparison to those fed R-VII, R-V, R-III, R-VI, R-II and R-I rations. Almost similar ADG was noticed in lambs fed R-III, R-V and R-VII rations and the supplementation of concentrate at 0.5%, 1.0% and 1.5% of body weight plus maize silage ad lib has increased the growth/gain in ram lambs by 62.48, 99.81 and 163.25 per cent, respectively in comparison to sole silage feeding. Similarly, supplementation of lucerne hay and groundnut haulms with maize silage has increased the weight gain by 109.31 and 84.97 per cent, respectively in comparison to sole maize silage fed animals. Sole sweet sorghum bagasse (SSB) based complete ration (50:50) fed lambs (R-VII ration) showed the ADG almost similar to those animals fed lucerne hay plus maize silage ad lib. Conclusions:Based on the results of this study, it is concluded that, maize silage can be supplemented either with lucerne hay, groundnut haulms (to meet 25% of DM requirement) or concentrate mixture at 1.5% of body weight for obtaining optimum growth rate in Nellore ram lambs.

A study was carried out to characterize and explore the expression profile of BMP 3 gene in control broiler and control layer chicken. The total open reading frame of BMP 3 (1389 bp) was cloned and sequenced. The control broiler and control layer chicken showed variation at nucleotide and amino acid level with reference gene (Gallus gallus, NCBI Acc. No. NM_001034819). When compared to reference gene, the control broiler showed four nucleotide differences (c.192A>G, c.519C>T, 903G>A and 960C>G), while, control layer showed variation at c.33G>C, 192A>G, 858G>A, 904G>A, 960C>G and 1257C>T making six differences in total. However, between control broiler and control layer lines, nucleotide differences was observed at c.33G>C, 519T>C, 858G>A, 903A>G, 904G>A and 1257C>T. The change at amino acid level between reference and control broiler was p.D320N and with control layer chicken, it was p.D302N and p.D320N. On the other hand, a single amino acid difference (p.D302N) was observed between the control broiler and control layer chicken lines. The phylogenetic study displayed a close relationship between broiler and layer lines and reference gene and also with other avian species resulting in a cluster formation. These cluster in turn displayed a distant link with the mammalian species. The expression profile of BMP 3 gene exhibited a variation at different stages of embryonic development and also at post embryonic period among the lines with control layer showing higher expression than that of broiler chicken. The protein was also detected in bone marrow tissue of broiler and layer lines by western blotting. It is concluded that the BMP 3 gene sequence differed at nucleotide and amino acid level among the lines and the gene expressed differentially at different periods of embryonic development and also at post hatch period.