Introduction: Sacoglottis gabonensis (Baille) Urban (Humiriaceae) is a medicinal plant used in the treatment of Buruli ulcer in Ivory Coast. To ensure its effect over a long period of use, the subchronic toxicity of the total aqueous extract of S. gabonensis stem bark (ETASg) in rats was evaluated. Methods: 80 rats were homogeneously distributed in 4 lots of 20 rats each, including 10 males and 10 females. ETASg was administered daily orally for 90 days for 2 mL/100 g body weight (b.w.) rats at doses of 3.5; 35 and 350 mg/kg b.w. The control group received distilled water. A venous blood sample is taken every 30 days for 90 days to determine serum biochemical parameters.

Blighia unijugata is a very widespread forest species in tropical Africa. It is usually used to treat various pathologies including hypertension. This study aims at evaluating the safety use of butanolic fraction of Blighia unijugata leaves (BuF) on some haematological parameters, body weight and relative organ weights in Wistar rats. Twenty-four adult male rats were randomly divided into four groups of six rats each. The control group was repeatedly administered by oral route with distilled water at 10 mL/kg bw for 28 days while test groups 2, 3 and 4 were repeatedly gavaged with BuF extract at 50, 500 and 1000 mg/kg bw respectively. Blood withdrawls were performed at days 7, 14, 21 and 28 using EDTA tubes by ocular puncture from fasted rats previously anesthetized with ether in order to assess BuF extract influence on some haematological parameters. At the end of the twenty-eight days of treatment, rats were weighed and sacrificed. Kidneys, liver, spleen and heart were removed and their relative organ weights were determined. The results showed that BuF extract induced a non-significant decrease (P>0.05) in some haematological parameters such as erythrocytes, haemoglobin, mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), leucocytes, lymphocytes, monocytes, granulocytes and significant (P<0.05) in hematocrit and thrombocytes in the treated rats compared to the control group. However, no significant changes (P>0.05) were observed in body weight and the relative organ weights such as heart, liver, kidneys and spleen during this study. This study indicates that butanolic fraction of Blighia unijugata leaves (BuF) administered to rats decreases hematocrit and thrombocyte levels at 50, 500 and 1000 mg/kg bw and is safe for other hematological parameters and body weight in rats.

Ageratum conyzoïdes (Aerial part) is a medicinal plant commonly used in traditional medicine in Côte d'Ivoire in the treatment of various diseases including bleeding. The objevctives of this study were investigated the hemostatic effects of A. conyzoïdes aqueous aerial part extract at differents volume in vitro, using blood samples from apparently healthy human volunteers. Its properties have been identified by hematologic tests such as coagulation time, prothrombin and activated cephalin time. The results showed that the aqueous extract of A. conyzoïdes (100 mg/mL) significantly (p < 0.001) reduced clotting time, prothrobin time, and activated cephalin time compared to the control (administered with only NaCl 0.9 %) in the blood obtained from the volunteers. In contrast, the aqueous extract did not influence thrombocytes count (p > 0.05). The aqueous leaf extract of A. conyzoïdes in a volume of 0.5 mL in this study had coagulation properties, which indicates the positive hemostatic effect on in vitro method. In cconclusion, this study justifies its use to traditionally stop bleeding.

Obesity is a chronic disorder of global prevalence and associated with morbidity and mortality. This pathology is a real public health problem. The work was undertaken to evaluate the antiobesity efficacy of aqueous extract of Kemite in cafeteria diet induced obese Wistar rats for a period of 28 days. Aqueous extract of Kemite (AEK) was prepared by hot extraction method. Female Wistar rats weighing 124-170 g were divided into different groups i.e. Normal control, cafeteria control and aqueous extract of Kemite at dose of 200 mg/kg bw. The antiobesity activity is estimated in terms of body weight gain, food intake, serum triglycerides (TG), Total cholesterol (TC), LDL cholesterol (LDL-C), HDL cholesterol (HDL-C), VLDL cholesterol (VLDL-C), blood glucose (BG), ASAT and ALAT activities, atherogenic index, coronary risk index and liver and fat pad weights. Results showed Cafeteria diet fed rats for 28 successive days significantly increased the body weight, food intake, ASAT and ALAT activities, liver and fat pad weights, atherogenic index, coronary risk index TG, TC, LDL, VLDL, BG and not influenced HDL levels. Rats treated with extract for 28 successive days along with cafeteria diet reversed the effects induced by cafeteria diet. In conclusion, this study revealed that AEK may be a natural and safe remedy for the prevention and control of obesity.

Sacoglottis gabonensis is a medicinal plant used traditionally for the treatment of Buruli ulcer in Côte d’Ivoire. To ensure its effect over a long period of use, assessment of the total aqueous stem bark extract of S. gabonensis (ETASg) on anatomo-histology of Wistar rat liver, kidneys and heart was evaluated. ETASg was administered to Wistar rats at doses of 3.5; 35 and 350 mg/kg/day for 90 days. The liver of rats treated with ETASg at 35 and 350 mg/kg showed color and appearance changes with whitish nodules. Furthermore, histological architecture revealed micro-vesicular steatosis. In contrast, gross examination did not showed any change color and appearance of the kidneys and heart of the rats tested at all doses of ETASg compared to those of control rats. Histological sections of the kidneys and heart of rats tested at all doses of ETASg showed normal kidney and heart cells. After stopping the administration, the liver abnormalities disappeared at the end of the 120 days. This study revealed that the integrity of the liver tissue is preserved only with ETASg of 3.5 mg/kg. On the other hand, it is guaranteed at all the doses studied for the renal and cardiac tissues.