Vitamin A deficiency is a major public health problem in Nigeria. Staple crops are targeted by biofortification efforts because they possess low micronutrient density and are consumed in large quantities by a large proportion of poor households. This study was designed to assess the knowledge, perception and utilization of biofortified cassava and orange-fleshed sweet potato in selected Local government Areas in Abia and Imo States. The study was descriptive and cross-sectional in design. A total of 710 respondents were selected using a multi-stage sampling technique. A structured interviewer-administered questionnaire was used to elicit the required information. Statistical analysis was performed using descriptive statistics(frequency, percentage, mean and standard deviation)and inferential(correlation)analysis. Socio-demographic data revealed a preponderance of females, married, well-educated and working-class respondents. Monthly income above ₦29,000(US$ 70.54) and household size of 4-6 members were reported in 53.5% and 48.6% of the respondents. Study results further revealed that some (34.9%)of the respondents had good knowledge, while 46.1% and 19.0% had an average and poor knowledge of biofortified staples, respectively. Orange fleshed sweet potatoes and biofortified cassava were purchased at least once monthly by 73.7% of the respondents, however 16.5% and 27.5% of the respondents consumed these biofortified staples on a daily and weekly basis. Low positive perception towards the consumption (27.5%), benefits (39.6%) and barriers (16.9%) of utilizing studied biofortified staples was reported. A positive correlation was observed between respondents’ knowledge of biofortified staples and their benefits perception (p = 0.003), purchase (p =0.001) and consumption (p= 0.001) frequency.Therefore, while a good number of the respondents were fairly knowledgeable about the studied biofortified staples, it significantly influenced the perception and utilization of these staples, despite being reportedly low. Therefore, increased sensitization, price subsidy and availability of these biofortified staples will help improvetheir acceptance and utilization among rural households.