During their feeding process, dung beetles perform a series of ecosystem functions that provide valuable ecosystem services, such as soil fertilization, improvement of soil properties, plant growth enhancement, and biological pest control. However, in the grasslands of the Central Asian dry steppe, the effects of dung beetles on dung removal remain almost unstudied. Here, we examined dung removal by different dung beetle species (Colobterus erraticus (Linnaeus, 1758), Onthophagus bivertex Heyden, 1887, Onthophagus gibbulus (Pallas, 1781), Gymnopleurus mopsus (Pallas, 1781), Cheironitis eumenes Motschulsky, 1859, and Geotrupes koltzei Reitter, 1892), and compared the impacts with control treatments (without beetles) under natural pasture conditions and in the laboratory. We examined the influence of different variables on dung removal rates, such as dung type and dung beetle traits (nesting strategies, abundance, body size, and biomass). We found higher dung removal rates during the initial 48 h in field and laboratory conditions. Among nesting strategies, tunnellers demonstrated significantly higher dung removal rates than dweller and roller species. The highest amount of dung removal was estimated for C. eumenes (6.5 g/day by seven individuals). We found no significant relationship between dung removal rates and dung beetle body size or biomass, but we observed a strong negative correlation between dung beetle abundance and dung removal rates. Our findings highlight the importance of dung type and age, nesting strategies and abundances of dung beetles, and experimental conditions, which are the main factors driving the process of dung removal.