Grain micronutrient content assessment is important in breeding pearl millet, in order to maintain or improve its high nutritional quality. Grain samples of 12 farmer-preferred pearl millet varieties produced in four representative environments in Niger during the 2013 rainy season were assessed for Fe, Zn, Al and P grain densities using energy dispersive X-ray fluorescence spectroscopy. Combined analysis of variance revealed significant (P < 0.01) variation among entries across environments only for Fe grain density, which had a broad-sense heritability of 89%. Fe grain density varied from 30.4 to 39.2 mg kg -1 among these varieties, with a mean of 32.4 mg kg -1. Among the 12 varieties, ICRI-Tabi and ICMV-IS 13131 (reselected ICRI-Tabi) showed the highest Fe grain densities.