Background & Method: The present study was conducted at Department of General Surgery, Index Medical College, Hospital & Research Center, Indore (M.P.). A total of 30 study subjects (post operated cases of urolithiasis) were selected from the surgical ward in Index Hospital from period of 12 months with an aim to Biochemical Analysis of Stones in cases of Urinary Tract Calculus. Result: A total of 46 renal stones were analyzed, from 30 patients. In 30 patients, 18 were Males and 12 Females. The Bio-chemical analysis of renal stones revealed the presence of mixed stones with highest present of Calcium Oxalate stones, followed by uric acid stones. Conclusion: This study demonstrated that in Index Medical College and Hospital a significant proportion of renal stones were containing calcium oxalate as constituent and were non phosphate in composition. The study concluded that the biochemical composition of renal calculi is important during the treatment of renal stone disease and reducing the recurrence by avoiding promoters of stone formation. Keywords: Biochemical, Stones, Urinary Tract & Calculus.