The essential oil (EO) of Cota tinctoria var. tinctoria was analyzed using GC-FID / MS. A total of 51 compounds were determined from this taxon, accounting for 99.79% in hydrodistillation. Monoterpenes were the primary chemical class for the volatile organic compounds in the EO (36.1%, 13 compounds). Borneol (18.1%), camphor (14.9%), and β -pinene (11.3%) were the major components in the EO of C. tinctoria var. tinctoria . The antimicrobial activities of EO and n- hexane, acetonitrile, methanol, and water solvent extracts of the taxon were screened in vitro against ten microorganisms. The EO yielded the best activity (15 mm, 372.5 MIC, 59600 μg/μL) against Mycobacterium smegmatis . The acetonitrile extract was the most active against the Staphylococcus aureus and Bacillus cereus with 274 μg/mL MIC value. IC 50 values for the lipase enzyme inhibitory activity of EO and solvent extracts ( n- hexane, acetonitrile, methanol, and water) were found to be 59.80 ± 4.3285 μg/mL 68.28 ± 3.1215 μg/mL, 52.60 ± 3.7526 μg/mL, 48.73 ± 2.8265 μg/mL, and 99.50 ± 5.5678 μg/mL, respectively.