Introduction:The vascular system is a vast network of vessels through which blood circulates in the body. Blood leaving the ventricles is distributed through arteries and arterioles in progressively smaller branches to the capillaries Deep vein thrombosis is a medical condition that occurs when a blood clot forms in a deep vein. A world wide survey conducted by CDC shows that 900,000 people could be affected by DVT (1 to 2 per 1,000) each year in the United States. Among people who have had a DVT, one-half will have long-term complications. DVT is a serious condition that can be life-threatening. However, it's largely preventable and treatable. Aim: To assess the knowledge and practice regarding prevention of deep vein thrombosis among bedridden patients. Materials and Methods: A cross-sectional study was carried out among the bedridden patients of Yenepoya Medical College Hospital, Mangalore. The content validity of the tool was established in consultation with 7 experts. The reliability of the tools were found to be r (6) = 0.8 and 0.7 which was statistically significant. The tools were found to be reliable. Non probability convenience sampling was used to select the subjects for the study. Pilot study was conducted to find out the feasibility of the study. Data collected from the 95 subjects were analyzed by descriptive and inferential statistics using SPSS (Version 23). Results: The findings of the study demonstrated that among 95 bedridden patients, that maximum percentage (61%)were belonged to the age group of 47 years and above. Majority (72%) were males. (41%) of were completed primary education. Majority (62%) of the respondents were physically active workers.58% of subjects had inadequate knowledge and 42% of subjects had moderate knowledge and none of the subjects had adequate knowledge on prevention of deep vein thrombosis and 22% of subjects had low practice, whereas majority 66% of respondents had moderate practice level and 12% of subjects had high practice level. Mean knowledge score was 11.14 and mean practice score was 9.85. There existed a positive relationship between knowledge and practice (correlation coefficient =0.662*) at 0.05 level of significance. The study also indicated that chi-square value of demographic variables have no significant association with knowledge scores and practice scores of bedridden patients.