Objective: The objective of this study was to evaluate whether a Class II type A2 biosafety cabinet in a laboratory could be decontaminated while the laboratory was being fumigated using vaporous hydrogen peroxide or peracetic acid dry fogging. Methods: To validate decontamination of all parts of the biosafety cabinet, biological indicators were placed at various locations within the biosafety cabinet, including between the pleats of supply and exhaust HEPA filters. To assess whether the operational status of the biosafety cabinet influenced the outcome of its decontamination, fumigation validations were undertaken with the cabinet running and not running. The amount of fumigant and the duration of fumigation remained constant whether the biosafety cabinet was running or not. Discussion: Biosafety cabinet decontamination was successful only when the cabinet was running to facilitate the fumigant’s circulation within the plenums and across the HEPA filters. This study shows both vaporous hydrogen peroxide and peracetic acid dry fogging can be used successfully to decontaminate Class II type A2 biosafety cabinets during laboratory fumigation, provided the biosafety cabinets are operational and running during the fumigation.

As you know, the future of the Delaware River, the longest free-flowing river in the eastern United States, is vital to the economy of the regions surrounding this important waterway. Wild and Scenic River designation will encourage natural and historic resource preservation and protect precious open space. By allowing local municipalities to sustain and protect the Delaware River as one of our nation's national treasures, this law will help to ensure the vitality of these communities and the quality of life of their citizens."-Former President Bill Clinton in a congratulatory letter to U.S. Rep. Rush Holt (D-N.J.)National TMDL Science and Policy