Despite a commitment by the European Union to protect its migratory bat populations, conservation efforts are hindered by a poor understanding of bat migratory strategies and connectivity between breeding and wintering grounds. Traditional methods like mark-recapture are ineffective to study broad-scale bat migratory patterns. Stable hydrogen isotopes (δD) have been proven useful in establishing spatial migratory connectivity of animal populations. Before applying this tool, the method was calibrated using bat samples of known origin. Here we established the potential of δD as a robust geographical tracer of breeding origins of European bats by measuring δD in hair of five sedentary bat species from 45 locations throughout Europe. The δD of bat hair strongly correlated with well-established spatial isotopic patterns in mean annual precipitation in Europe, and therefore was highly correlated with latitude. We calculated a linear mixed-effects model, with species as random effect, linking δD of bat hair to precipitation δD of the areas of hair growth. This model can be used to predict breeding origins of European migrating bats. We used δ13C and δ15N to discriminate among potential origins of bats, and found that these isotopes can be used as variables to further refine origin predictions. A triple-isotope approach could thereby pinpoint populations or subpopulations that have distinct origins. Our results further corroborated stable isotope analysis as a powerful method to delineate animal migrations in Europe.

Aim  We investigate the population genetic structure of the Maghrebian bat, Myotis punicus, between the mainland and islands to assess the island colonization pattern and current gene flow between nearby islands and within the mainland. Location  North Africa and the Mediterranean islands of Corsica and Sardinia. Methods  We sequenced part of the control region (HVII) of 79 bats across 11 colonies. The phylogeographical pattern was assessed by analysing molecular diversity indices, examining differentiation among populations and estimating divergence time. In addition, we genotyped 182 bats across 10 colonies at seven microsatellite loci. We used analysis of molecular variance and a Bayesian approach to infer nuclear population structure. Finally, we estimated sex‐specific dispersal between Corsica and Sardinia. Results  Mitochondrial analyses indicated that colonies between Corsica, Sardinia and North Africa are highly differentiated. Within islands there was no difference between colonies, while at the continental level Moroccan and Tunisian populations were highly differentiated. Analyses with seven microsatellite loci showed a similar pattern. The sole difference was the lack of nuclear differentiation between populations in North Africa, suggesting a male‐biased dispersal over the continental area. The divergence time of Sardinian and Corsican populations was estimated to date back to the early and mid‐Pleistocene. Main conclusions  Island colonization by the Maghrebian bats seems to have occurred in a stepping‐stone manner and certainly pre‐dated human colonization. Currently, open water seems to prevent exchange of bats between the two islands, despite their ability to fly and the narrowness of the strait of Bonifacio. Corsican and Sardinian populations are thus currently isolated from any continental gene pool and must therefore be considered as different evolutionarily significant units (ESU).

Previous genetic analyses have demonstrated that two divergent lineages of Pipistrellus kuhlii are spread over Europe and North Africa, and it has been proposed that Pipistrellus maderensis, a taxon endemic to the Canary Archipelago and Madeira, was its sister species. In this study, we used mitochondrial DNA sequences to investigate the level of endemism achieved by Corsican lineages with regard to their continental counterparts and to propose hypotheses about the geographical origin of Corsican bats. Our results suggest that Corsican Kuhl's pipistrelles are not endemic. Such a lack of genetic endemism in Corsica can result from current gene flow with French and Italian populations and/or recent colonization of this island. Additionally, our results demonstrate that Corsica was colonized independently from Europe by two divergent lineages (genetic distance= 5.8%) widespread in the western Palaearctic and clearly suggest that North Africa probably does not play any significant role in the colonization of Corsica by the Kuhl's Pipistrelle. Additional morphometric, acoustic and ecological studies are needed to soundly ascertain the respective taxonomic status of these two divergent lineages and the level of distinctiveness achieved by Corsican bats.

The presence of the Maghrebian bat,

We present a recent survey of the vertebrate fauna of Cavallo, a small island belonging to the Lavezzi archipelago located between Corsica and Sardinia. This island is the only inhabited one off the coast of Corsica and underwent deep landscape and ecosystem changes in the past 50 years induced by touristic development. The herpetofauna of the Cavallo island has changed with two recent colonisations, one green frog and one gecko. Two amphibian species and four reptiles live nowadays on this island. Concerning the breeding landbirds, this island has been visited by ornithologists more or less regularly since 1955. Several species have colonized the island while others have left. These changes are due to important modifications of the habitats, such as urbanization, the decline of high matorral and grasslands, but also the development of gardens and the creation of a permanent wetland. Today, 13 species of landbirds breed on Cavallo. Several introduced mammal species recorded in the 1980s seem to have vanished, and today the list is restricted to the introduced Black rat and two or three bat species.