Coliphages T I and T? were efficiently adsorbed from dialyzed lysates by small columns of calcium phosphate. Elution of the columns with phosphate buffers a t low ~nolarity (0.02-0.03) removed much of the non-infectious material; the phage could subsequently be eluted ill more concentrated and purified form with higher buffer concentrations (0.06 ill or greater). The chromatographic behavior of T I and TI is contrasted and the influence of non-phage constituents of the lysate upon the chro~natographic separation is discussed.
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