A biocomposite material of hyaluronic acid (HA) and room temperature ionic liquid, 1-butyl-3-methyl-imidazolium tetrafluoroborate (BMIMBF 4 ) was fabricated and characterized by voltammetry and electrochemical impedance spectroscopy. Hemoglobin (Hb), as a model protein, was immobilized in this biocomposite on glassy carbon electrode to investigate its direct electrochemistry. A pair of well-defined, quasireversible cyclic voltammetric peaks appeared with the formal potentials (E8') at À 0.360 V (vs. SCE). The Hb modified electrode exhibited an excellent electrocatalytic activity to the reduction of H 2 O 2 with a wide linear range, good selectivity, sensitivity and reproducibility, which had potential application to fabrication the third-generation biosensor.

No abstract