No abstract

The first several months of life are a critical period for neuronal plasticity in the visual cortex during which anatomic and physiological development depends on visual experience. In cats, electrophysiologically assessed neuronal plasticity is minimal until approximately 3 weeks, peaks at 5 weeks, gradually declines to low levels at 20 weeks, and disappears at approximately 1 year of age (Daw, 1994). Rearing in darkness slows the entire time course of this critical period, such that at 5 weeks of age, normal cats are more plastic than dark-reared cats, whereas at 20 weeks, dark-reared cats are more plastic (Mower, 1991; Beaver et al., 2001). Thus, a stringent criterion is that genes that are important for plasticity in visual cortex will show differences in expression between normal rearing and dark rearing that are of opposite direction in young versus older animals. The present study reports the identification by differential display PCR of Munc13-3, a mammalian homolog of the Caenorhabditis elegans "uncoordinated" gene (unc-13), as a candidate gene for critical-period neuronal plasticity, the expression of which is regulated according to this criterion specifically in visual cortex and not in frontal cortex. Other members of the Munc13 family (Munc13-1 and Munc13-2) do not meet this criterion in visual cortex, indicating that Munc13-3 is the only family member that is regulated by age and dark rearing in the same manner as physiological plasticity during the visual cortical critical period.

Lactic acid bacteria (LAB) are the main microorganisms used as probiotics against gastrointestinal inflammation. The objective of this study was to evaluate the potential probiotic characteristics (antimicrobial activity, artificial gastrointestinal model resistance, cell surface hydrophobicity, and autoaggregation ability) and safety characteristics (hemolytic activity, antimicrobial resistance, and in vivo safety) of LAB isolated from the rumen fluid of an adult female sika deer. Two isolated strains identified as Latilactobacillus curvatus Z12 and Z19 showed good antimicrobial activity against enteropathogenic Escherichia coli (ATCC25922), Salmonella typhi (ATCC14028), and Staphylococcus aureus (ATCC25923). In addition, L. curvatus Z12 exhibited higher artificial gastrointestinal model resistance, cell surface hydrophobicity and autoaggregation ability than L. curvatus Z19. Therefore, regarding safety characteristics, only L. curvatus Z12 was evaluated. Upon assessment of safety, L. curvatus Z12 was negative for hemolytic activity and susceptible to penicillin G and cefamandole. Furthermore, an in vivo safety assessment showed that high-dose L. curvatus Z12 (109 CFU/mL) supplementation not only had no adverse effects on body weight gain, feed intake, and organ coefficients of treated mice but also played a key role in promoting the immune system maturation of treated mice. This research revealed that L. curvatus Z12 possesses desirable probiotic characteristics and could be used as a potential probiotic feed additive to improve sika deer health.

In this study, Mg2Si in in-situ Mg2Si/Al composites were subjected to modification treatment using rare earth element Holmium (Ho). The phase composition and microstructure before and after modification of Mg2Si were also analyzed using X-ray diffraction (XRD) and optical microscope (OM); in addition, its mechanical properties were detected as well. The results showed that moderate addition of rare earth element Ho in in-situ Mg2Si/Al composites presents good modification effects on Mg2Si, the morphology of primary Mg2Si was changed from cross shape before modification to dispersed irregular mass; optimal modification effect was obtained when 0.4% rare earth element Ho was added. Under this condition, the average size of primary Mg2Si was decreased from 74 μm before modification to 16 μm; its mechanical properties were promoted significantly; and its tensile strength increased from 134 MPa before modification to 174.6 MPa, its brinell hardness elevated from 76 HB to 90 HB.