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For automating the harvesting of bunches of tomatoes in a greenhouse, the end-effector needs to reach the exact cutting point and adaptively adjust the pose of peduncles. In this paper, a method is proposed for peduncle cutting point localization and pose estimation. Images captured in real time at a fixed long-distance are detected using the YOLOv4-Tiny detector with a precision of 92.7% and a detection speed of 0.0091 s per frame, then the YOLACT +  + Network with mAP of 73.1 and a time speed of 0.109 s per frame is used to segment the close-up distance. The segmented peduncle mask is fitted to the curve using least squares and three key points on the curve are found. Finally, a geometric model is established to estimate the pose of the peduncle with an average error of 4.98° in yaw angle and 4.75° in pitch angle over the 30 sets of tests.

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