The medicinal plants of the genus Argemone spp are used for the alternative treatment of various pathologies and have a wide distribution in the Mexican territory. However, phytochemical knowledge about the genus Argemone spp is limited, and the scientific literature is scarce. Therefore, in the present work, we aimed to investigate the chemical composition, antioxidant, toxicological, and antimicrobial properties of Argemone spp. Ethanolic and acetic acid extracts of the different vegetal tissue parts of the plant were obtained. The Higher content of phenols than flavonoids in acetic and ethanolic extracts in petals and sexual organs. While the higher amount content of total alkaloids in the Acetic acid extracts of the leaf, stem, and sexual organs. Phenols and total flavonoids obtained from ethanol extracts showed a higher antioxidant activity than acetic extracts. However, lower toxicity in the Acetic acid extracts was found. The data show that ethanolic seed extracts are a good source of compounds with antifungal activity (C. albicans). In contrast, acetic Acid extracts of different tissues except seeds are a good source of secondary metabolites mostly with anti-bacterial properties.