Natural colorants are important alternatives to synthetic colorants. They are considered harmless and positively affect biological activities owing to their antioxidant potential. The present study deals with the assessment of the extraction processes and the effects of pH (1.0, 3.0, and 5.0), extraction media (water and 50% ethanol) and storage condition (ambient and refrigeration) on betacyanin content, color values, as well as degradation kinetics of total betacyanins in red amaranth. Betacyanin content was more stable at higher than at lower pH. The degradation rate constant (K) was higher and the half-life (t 1/2) was lower at ambient temperature compared to refrigeration temperature. Betacyanin degradation was higher at ambient temperature (30 ± 2°C) than at refrigeration temperature (4°C). The pH, storage time, and temperature affected the stability of the color attributes. Therefore, this work suggests that water and lower temperature (4°C) could be applied to extract more betacyanins from red amaranth and betacyanins might be used as an alternative to synthetic color.
Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disease that can lead to carcinoma, cirrhosis, and death. Since no approved medications are available, dietary interventions that include bioactive compounds have been recommended. This study investigated the effects of black ginseng extracts (BGE) and aged black garlic extracts (AGE) on high‐fat diet (HFD)‐induced obese mice. Micrograph of liver tissues of mice fed with BGE and AGE showed less lipid droplets. The BGE and AGE supplements individually and in combination lowered the marker enzymes, aminotransferase (AST), and alanine aminotransferase (ALT) levels indicating their hepatoprotective effects. Compared to the plants extracts alone, the combination of the extracts resulted in lower total cholesterol (TC) and low‐density lipoproteins cholesterol (LDL‐C), which are risk markers for cardiovascular morbidity and mortality. Diets with the combination of BGE and AGE supplements had higher superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px) activities, and lower malondialdehyde indicating the synergistic effects of the extracts. Irrespective of the diet type, all treated groups showed lower tumor necrosis factor (TNF‐α) values as compared to HFD, which indicated overall immunomodulatory effect of both extracts. Therefore, the innovative formulation formed by the combination of BGE and AGE can provide hepatoprotective effects via modulating glycometabolism, lipometabolism, oxidative stress, and inflammation in mice.
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