Citrus essential oils have become the focus of several researches, because they have broad biological activity, due to their chemical composition. However, there are few studies covering Moro orange essential oil. Thus, the aim of this study was to evaluate the effects of fresh Moro orange peel essential oil on antifungal, antioxidant, cytotoxic, and genotoxic activities in normal and tumor cells. The essential oil was extracted by hydrodistillation and characterized by GC/MS and GC/FID. Cytotoxicity was evaluated by MTS assay in normal cells (CCD‐1059Sk) and tumor cells (CCD‐1059Sk), and the genotoxicity was determined in normal cells by the comet assay. Antifungal activity was evaluated by the disk diffusion test against Aspergillus carbonarius and Aspergillus flavus filamentous fungi. The antioxidant activity was determined in DPPH, ABTS, and β‐carotene assay. The essential oil was mainly composed of monoterpenes such as limonene (95.12%), α‐pinene (0.35%), sabinene (0.54%), and myrcene (1.07%). The essential oil did not cause cell death after 48 hours of treatment in normal cells, but the cell viability of tumor cells decreased by 50% in the presence of the essential oil at a concentration of 272.6 μg/mL. Although no antioxidant effect was observed, the growth of all evaluated fungi was inhibited with a minimum inhibitory concentration (MIC) of 125 μL/mL. The essential oil from the Moro orange is promising for cancer treatment, and it can be used as a natural preservative in food systems.

Schinus terebinthifolius Raddi is a tree present in Latin America, mainly in Brazil. The essential oils obtained from its leaves (LEO) and fruits (FEO) were evaluated for chemical composition cytotoxic, genotoxic and antifungal activities. The extraction of the essential oils was accomplished by the hydrodistillation technique. The characterization and quantification of the constituents were performed by gas chromatography coupled to a mass spectrometry detector and gas chromatography coupled to a flame ionization detector, respectively. The cytotoxic assay using tumor cells (lung adenocarcinoma, breast carcinoma, and melanoma) and normal cells was determined by the MTS assay. Genotoxic potential on normal cells was evaluated by Cometa assay. The analysis of antifungal activity was performed by evaluating the inhibitory effect on the growth of the Aspergillys carbonarius and Aspergillus flavus filamentous fungi using the disc diffusion test. The LEO contains limonene, D-germacrene, β-trans-caryophyllene, bicyclogermacrene, α-epi-murolol and α-copaene as the principal constituents, whereas the major constituents of FEO are myrcene, Δ-3-carene, β-eudesmol and α-phellandrene. Regarding cytotoxic activity on tumor cells, FEO was more effective than LEO. The IC50 values found for FEO on tumor cells varied between 244-302 μg.mL-1 compared to 653.4 ± 1.11 for normal cells. LEO was more cytotoxic against normal cell than tumor. Neither FEO nor LEO induced DNA damage at tested experimental conditions. A Minimum Inhibitory Concentration of 125 μL.mL-1 of both essential oils was determined for the fungi analyzed. It is inferred that these essential oils can be used in drug formulations when used in the correct concentration