Circular RNA-lipoprotein receptor 6 (circ-LRP6) serves a role in promoting the tumorigenesis of retinoblastoma, esophageal squamous cell cancer and oral squamous cell carcinoma; however, whether circ-LRP6 demonstrates the same effect in osteosarcoma (OS) is yet to be fully elucidated.The present study aimed to analyze the expression, role and potential molecular mechanism of circ-LRP6 in OS. The expression levels of circ-LRP6, microRNA (miR)-141-3p, histone deacetylase 4 (HDAC4) and high mobility group protein 1 (HMGB1) were evaluated by reverse transcriptionquantitative PCR in OS tissues and cell lines. Cell Counting Kit-8, Transwell and Matrigel assays were conducted to evaluate cell proliferation, migration and invasion, respectively. Western blotting was also performed to determine HDAC4 and HMGB1 protein expression levels. Bioinformatics and dual-luciferase reporter assays were used to predict and analyze the interactions between circ-LRP6 and miR-141-3p, miR-141-3p and HDAC4, as well as between miR-141-3p and HMGB1. Additionally, RNA immunoprecipitation was performed to verify the association between circ-LRP6 and miR-141-3p. The results confirmed that circ-LRP6 was highly expressed in OS tissues and cell lines. In addition, circ-LRP6 negatively regulated the expression of miR-141-3p and, in turn, miR-141-3p negatively regulated HDAC4 and HMGB1 expression. Functional assays revealed that circ-LRP6 knockdown inhibited the proliferation, migration and invasion of OS cells, whereas the inhibition of miR-141-3p or the overexpression of either HDAC4 or HMGB1 partly reversed the inhibitory effect of circ-LRP6 knockdown. In summary, the present study determined that circ-LRP6 knockdown inhibited the proliferation, migration and invasion of OS cells by regulating the miR-141-3p/HDAC4/HMGB1 axis.

Purpose The purpose of this study was to evaluate the ability of metagenomic next-generation sequencing (mNGS) diagnosing prosthetic joint infection (PJI) using tissue from hip/knee rapidly and precisely, especially in patients who had received antibiotic treatment within the preceding two weeks. Methods From May 2020 to March 2022, 52 cases with suspected PJI were enrolled. mNGS was performed on surgical tissue samples. The sensitivity and specificity of mNGS in diagnosis was evaluated using culture in conjunction with MSIS criteria. This study also looked at how antibiotic use affected culture and mNGS efficacy. Results According to MSIS criteria, 31 of the 44 cases had PJI, and 13 were classified in the aseptic loosening group. Sensitivity, specificity, positive/negative predictive value (PPV/NPV), positive/negative likelihood ratio (PLR/NLR), and area under the curve (AUC) of mNGS assay were 80.6% (71.9–91.8%), 84.6 (73.7–97.9%), 92.6 (84.2–98.7%), 64.7 (58.6–74.7%), 5.241 (4.081–6.693), 0.229 (0.108–0.482) and 0.826 (0.786–0.967), respectively, with MSIS as a reference. When MSIS was used as a reference, the results of culture assay were 45.2% (40.8–51.5%), 100 (100.0–100.0%), 100 (100.0–100.0%), 43.3 (39.1–49.5%), +∞, 0.548 (0.396–0.617) and 0.726 (0.621–0.864), respectively. The AUC values for mNGS and culture were 0.826 and 0.731, respectively, and the differences were insignificant. mNGS demonstrated higher sensitivity than culture in PJI subjects who had previously received antibiotic treatment within 2 weeks (69.5% vs 23.1%, P = 0.03). Conclusion In our series, mNGS yield a higher sensitivity for diagnosis and pathogen detection of PJI compared to microbiological culture. Additionally, mNGS is less affected by prior antibiotic exposure.

We first derive an integrable deformed hierarchy of short pulse equation and their Lax representation. Then we concentrated on the solution of integrable deformed short pulse equation (IDSPE). By proposing a generalized reciprocal transformation, we find a new integrable deformed sine-Gordon equation (IDSGE) and its Lax representation. The multisoliton solutions, negaton solutions and positon solutions for the IDSGE and the N-loop soliton solutions, N-negaton and N-positon solutions for the IDSPE are presented. In the reduced case the new N-positon solutions and N-negaton solutions for short pulse equation are obtained. 1 et al. proposed the integrable semi-discrete and full-discrete analogues of the SPE. As far as the solutions of the SPE are considered, in [9] not only solitary wave solutions are obtained by making use of transformation between the SPE and sine-Gordon equation but also the pulse solutions of the SPE were derived from the breather solutions of the SG equation. Some periodic and traveling wave solutions of SPE are given in [10]. Kuetche et al. constructed the two loop soliton solutions with use of bilinear method and hodograph transformation [11]. In [12,13], Matsuno develop a systematic procedure to construct the periodic solutions and multiloop solitons.The integrable deformation of integrable system attract a lot of interests from both physical and mathematical points. One kind of the integrable deformation is the socalled soliton equation with self-consistent sources [14]- [21], which consists of the soliton equation with additional terms by coupling the corresponding eigenvalue problems, and has important application. For example, the nonlinear Schrödinger equation with selfconsistent sources is relevant to some problems of plasma physical and solid state physics.In this paper, we consider the integrable deformed short pulse equation (IDSPE) which has not been studied yet. First we derive the integrable deformed hierarchy of short pulse equation(IDSPH) and their Lax representation, which includes integrable deformed short pulse equation (IDSPE) and its Lax representation. This implies that the IDSPE is Lax integrable. A generalized reciprocal transformation for the IDSPE is proposed. This transformation converts the IDSPE and its Lax representation into new integrable deformed sine-Gordon equation (IDSGE) and its Lax representation. The IDSGE can be written as bilinear form by introducing an independent variable transformation. The N-soliton solutions of the sine-Gordon equation were obtained in [22,23], and the positon solutions of sine-Gordon equation and its properties were studied in [23]. Here, we find the N-soliton solutions, N-negatons solutions and N-positon solutions for the IDSGE . Further using the inverse reciprocal transformation, we construct N-loop soliton solutions, N-negaton solutions and N-positon solutions of the IDSPE. In the reduced case we obtain the new N-negaton solutions and new N-positon solutions for the short pulse equation.This paper is organized as follows. In s...