Background and aims: This study aims to investigate the effects of muscle relaxation exercise and music therapy applied before the drain removal operation on pain, vital signs, and psychometric variables. Methods: This study was carried out with 90 volunteer patients (45 experiment, 45 control group) between May 2014 and November 2014. Data were collected using the Patient Identification Forms, the Visual Analogue Scale (VAS) and Vital Signs forms with the face-to-face interviews. For the control group, the VAS scores and vital signs were noted and evaluated 15 minutes before the drain removal, immediately after the drain removal and at 15 min. The Music (Rast Mode), which was selected 15 minutes before the operation was listened by the experiment group patients, while muscle relaxation exercise was instructed and performed. Results: In the experimental group, 53.3% were women, 61.4% were married and 28.9% were between 46 and 60 years of age. In the control group 62.2% were women, 82.2% were married and 33.3% were over 61 years of old. Compared to the control group, the VAS scores and systolic blood pressure increased immediately after the drain removed in the experiment group, falling below the initial value at 15 min. In terms of the type of the drain used, the number, diastolic pressure, and pulse rate, no relationship was found between the two groups (p>0.05). Conclusion: During the removal of drains, nurses play an important role in the practice of nonpharmacological methods, and may prevent the unnecessary use of analgesics. Muscle relaxation exercises and music therapy should be applied prior to drain removal procedures, as effective non-pharmacological methods for pain control, and health personnel should have sufficient expertise regarding the importance of nonpharmacological methods in pain management.