Treatment of disseminatedTrichosporon infections still remains difficult. Amphotericin B frequently displays inadequate fungicidal activity and echinocandins have no meaningful antifungal effect against this genus. Triazoles are currently the drugs of choice for the treatment of Trichosporon infections. This study evaluates the inhibitory and fungicidal activities of five triazoles against 90 clinical isolates of Trichosporon asahii. MICs (g/ml) were determined according to Clinical and Laboratory Standards Institute microdilution method M27-A3 at 24 and 48 h using two endpoints, MIC-2 and MIC-0 (the lowest concentrations that inhibited ϳ50 and 100% of growth, respectively). Minimum fungicidal concentrations (MFCs; g/ml) were determined by seeding 100 l of all clear MIC wells (using an inoculum of 10 4 CFU/ml) onto Sabouraud dextrose agar. Time-kill curves were assayed against four clinical T. asahii isolates and the T. asahii ATCC 201110 strain. The MIC-2 (ϳ50% reduction in turbidity compared to the growth control well)/MIC-0 (complete inhibition of growth)/MFC values that inhibited 90% of isolates at 48 h were, respectively, 8/32/64 g/ml for fluconazole, 1/2/8 g/ml for itraconazole, 0.12/0.5/2 g/ml for voriconazole, 0.5/2/4 g/ml for posaconazole, and 0.25/1/4 g/ml for isavuconazole. The MIC-0 endpoints yielded more consistent MIC results, which remained mostly unchanged when extending the incubation to 48 h (98 to 100% agreement with 24-h values) and are easier to interpret. Based on the time-kill experiments, none of the drugs reached the fungicidal endpoint (99.9% killing), killing activity being shown but at concentrations not reached in serum. Statistical analysis revealed that killing rates are dose and antifungal dependent. The lowest concentration at which killing activity begins was for voriconazole, and the highest was for fluconazole. These results suggest that azoles display fungistatic activity and lack fungicidal effect against T. asahii. By rank order, the most active triazole is voriconazole, followed by itraconazole ϳ posaconazole ϳ isavuconazole > fluconazole.

No abstract

No abstract

No abstract

Bir eğitim ve araştırma hastanesinde artan sıklıkta izole edilenCorynebacterium striatum izolatlarının değerlendirilmesi Corynebacterium striatum strains are members of the normal flora of skin and mucous membranes, and commonly found in the environment. They have been regarded as contaminant for a long time when they reproduce in microbiological cultures. Lately, increase in the number of immunosuppressive patients and invasive procedures, and more frequent use of wide-spectrum antibiotics caused an increase in the number of reported infections and epidemics related to C. striatum strains. In this study, C. striatum isolates which are regarded as the infectious agents according to the microbiological criteria and their susceptibility to antibiotics were evaluated in our hospital for the last five years period and it was tried to attract the attention to this opportunistic pathogen having rapidly increasing isolation frequency. Methods:Various clinical samples sent to our hospital's Medical Microbiology Laboratory were Gram stained, and cultured in appropriate agars. The strains with leucocytes and/or bacteria on Gram stain, dominant or absolute growth in culture, and growth in the repeated cultures were regarded as infectious agents while bacterial growths not fulfilling those criteria were reported as contamination or colonization. Identification of the agents at stain level was performed with Vitek 2 (bioMerieux, France) system between 2010 and 2013, and with Bruker Microflex MS (Bruker Daltonics, Bremen, Germany) system in 2014. Results:The numbers of C. striatum strains that were regarded and reported as infectious agents Bulgular:Mikrobiyolojik kriterlere göre etken olarak kabul edilip raporlanan C. striatum izolatlarınınMakale Dili "Türkçe"/Article Language "Turkish"