Background: Diabetes mellitus, a group of metabolic disorders with multiple etiologies is characterized by chronic hyperglycaemia, induced by deficiency of insulin production and insulin action. Prediabetes is a type of hyperglycemia in which glycemic indices are higher than normal but lower than the diabetes threshold.Objective: This study aims to assess the impact of bone markers (serum calcium, phosphorus and alkaline phosphatase) on prediabetic and type 2 diabetes mellitus patients. Material and Methods: This study was carried out in 2021 from January to December, in the Department of Biochemistry, Teerthanker Mahaveer Hospital, and Moradabad. Comparative levels of serum calcium, serum phosphorus and alkaline phosphatase between 65 Prediabetic and 65 Type2 diabetes mellitus patients were measured using an automated method. Results: Serum ALP and serum phosphorus were significantly higher in the diabetic patients than that in the pre diabetic group (p<0.001), whereas serum calcium was significantly lower in type 2 diabetes mellitus patients than that in prediabetes (p<0.001). Conclusion: Our findings depict variations in serum mineral levels are linked to prediabetes and these parameters can be considered as independent predictors for the diagnosis of type II diabetes mellitus, and their early diagnosis could be beneficial to prevent further complications.