Low back pain is an occupational disease that arises due to work relationships or is caused by work and work attitudes. Incorrect sitting posture and sitting for long periods are one of the most frequent risk factors. The study was conducted to determine the relationship between sitting posture and work duration with complaints of low back pain in-office employees. The research design used is descriptive-analytic with a cross-sectional approach. The number of samples was 70 employees of the Central Kalimantan Provincial Health Office using the total sampling technique. The research data was taken using a questionnaire. The results of respondents who complained of low back pain were 12 people (17.1%), and 9 of them related to the wrong sitting position (81.8%) with a p-value of 0.000, and the wrong sitting posture was 84 times greater for suffering from lower back pain compared to the correct sitting posture. The duration of work (long sitting) did not have a relationship with complaints of back pain (p = 0.974). There is a significant relationship between the respondent's sitting attitude to the incidence of low back pain, and there is no significant relationship between work duration (length of sitting) and the incidence of low back pain in employees at the Central Kalimantan Provincial Health Office. Keywords: Work Duration, Sitting Length, Lower Back Pain, Sitting Position