The study documented the nature and locations of several previously unknown posttranslational modifications in lens fiber connexins. This detailed knowledge of the specific posttranslationally modified sites will allow further work to elucidate the mechanisms that different signaling pathways use to regulate connexins in lens fiber cells.

The connexins constitute a family of proteins that make up the intercellular membrane channels of gap junctions. We had previously reported the presence of two members of this protein family, connexins 32 and 43, in mouse one-cell zygotes (Barron et al., Dev Genet 10:318-323, 1989; Valdimarsson et al., Mol Reprod Dev 30:18-26, 1991), implying that both must be present in the mature oocyte and could be involved in mediating the intercellular coupling that occurs between the oocyte and cumulus granulosa during oogenesis. In the present report we provide evidence for this, based on an analysis of the cumulus-oocyte complex (COC) using reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry with a confocal microscope. Transcripts of both connexin32 (Cx32) and connexin43 (Cx43) were detected by RT-PCR in both components of the COC. Cx32 mRNA in the oocyte declined precipitously following human chorionic gonadotropin (hCG) stimulation of pregnant mare serum gonadotropin (PMSG)-primed ovaries, whereas there was no obvious change in Cx43 mRNA. Peptide-specific antibodies against both connexins provided diffuse cytoplasmic staining of oocytes as well as some punctate staining near the oocyte surface, which could not be unequivocally resolved as cumulus-oocyte gap junctions. However, the two antibodies did provide clear evidence of Cx32 and Cx43 in gap junction-like structures between cumulus cells. We could find no evidence of the incorporation of the oocyte's store of Cx32 into gap junctions during postfertilization development.(ABSTRACT TRUNCATED AT 250 WORDS)

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