We have isolated cDNA clones for the human antileukoprotease HUSI-I, an elastase inhibitor, from a library, containing cDNA inserts made from human cervix uterus. A library of 10000 recombinants was screened using a mixture of 16 different oligodeoxyribonucleotides which correspond to amino acids 79 -84 and one 20mer oligodeoxyribonucleotide corresponding to amino acids 19 -26. Two overlapping cDNA clones, containing the entire coding sequence and part of the 5'-and 3'-untranslated region, were isolated. DNA sequence data showed that our clone corresponds with the available protein sequence data. For expression, the cDNA fragment was inserted in a derivative of plasmid pPLc236 and expressed under the control of APL promoter. Expression of antileukoprotease was proven by Western blot analysis and inhibition of chymotrypsin.Neutral granulocytic proteinases as well as kallikreins are known to be potent mediators of the inflammatory response following polytraumatic injuries [I, 21. In vivo inhibition of these proteinases by low-molecular-mass inhibitors could serve as a valuable tool to decrease the inflammatory reaction [l, 3,4]. Human mucous fluids such as seminal plasma, cervical mucus, tears, bronchial and nasal secretions contain acidstable proteinase inhibitors with strong affinity for trypsin and chymotrypsin as well as for neutrophil lysosomal elastase and cathepsin G [5, 61. These inhibitors are characterized by their acid stability and low molecular mass. The antileukoprotease from human seminal plasma HUSI-I (human seminal plasma inhibitor I) and the inhibitor found in mucous secretions, from the cervix uteri, although isolated from different tissues seem to be identical proteins based on shared characteristics of acid stability and low molecular mass [I], partial amino acid sequence homology [7], inhibition spectrum and immuno-cross-reactivity [S]. We shall therefore refer to them jointly as ALP (antileukoprotease). The isolation of the inhibitor from human seminal plasma is complicated
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.