Crustacean hyperglycemic hormone (CHH) was isolated from sinus glands of Carcinus maenas, and its primary structure was determined by manual microsequencing, using the DABITC-PITC double-coupling method. The neurohormone consists of 72 amino acid residues (8524 Da). Three disulfide bridges are present and both the N-and C-terminus are blocked.CHH does not show significant sequence homology to any known peptide hormone or protein.Amino acid sequence; Crustacean hyperglycemic hormone; Neuropeptide; (Carcinus maenas)

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Locust adipokinetic hormones I (ΑΚΗ I) and II (ΑΚΗ II) are separated by reversed-phase high-performance liquid chromatography using a μ-Bondapak phenyl column with a trifluoroacetic acid/acetonitrile gradient. The eluant was monitored at 210 nm and the hyperlipaemic activity was detected using a bioassay.The amino-acid composition of ΑΚΗ II was determined after acid hydrolysis with HC1 or methanesulfonic acid. It contained the following aminoacid residues in almost equimolar amounts: Asp, Ser, Glu, Gly, Ala, Leu, Phe and Trp.Isolierung der adipokinetischen Hormone I und II der Wanderheuschrecke, Locusta migratoria, in nur einem Reinigungsschritt mittels Hochleistungsfl ssigkeitschromatographie und Aminos urezusammensetzung des adipokinetischen Hormons II Zusammenfassung: Die adipokinetischen Hormone I und II aus der Wanderheuschrecke wurden durch Hochleistungsfl ssigkeitschromatographie auf einer μ-Bondapak-Phenyl-S ule mittels eines Trifluoressigs ure/Acetonitril-Gradienten getrennt. Die Detektion erfolgte bei 210 nm. Die adipokinetische Aktivit t wurde in einem biologischen Test bestimmt.Die Aminos urezusammensetzung des adipokinetischen Hormons II erfolgte nach Hydrolyse in HG oder Methansulfons ure. Die folgenden Aminos urereste konnten ann herungsweise in quimolaren Konzentrationen bestimmt werden: Asp, Ser, Glu, Gly, Ala, Leu, Phe und Trp.Key words: Reversed-phase high-performance liquid chromatography, locust adipokinetic hormone I and II, amino-acid composition of locust adipokinetic hormone II.Locust corpora cardiaca contain an adipokinetic hormone (AKH)l 1 » 2 ! which mobilises specific diacylglycerols during flight^ and stimulates their oxidation by the flight muscles^4!. ΑΚΗ has been characterised as a blocked decapeptidel 5 ! and the structure confirmed by synthesis^6!. Recentlyi ? l, a second peptide (ΑΚΗ II) exhibiting adipokinetic activity in locusts and red pigment-concentrating hormone activity in shrimps has been found in locust corpora

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