Background In prenatal diagnosis, CMA has begun to emerge as a favorable alternative to karyotype analysis, but it could not identify balanced translocations, triploidies, inversion and heteromorphisms. Therefore, conventional cytogenetic and specific staining methods still play an important role in the work-up of chromosome anomaly. This study investigated the application of C-banding and AgNOR-staining techniques in prenatal diagnosis of chromosomal heteromorphisms and some structure abnormalities. Results Among the 2970 samples, the incidence of chromosomal heteromorphisms was 8.79% (261/2970). The most frequent was found to be chromosome Y (2.93%, 87/2970), followed by chromosome 1 (1.65 %, 49/2970), 9 (1.52 %, 45/2970), 22 (0.77 %, 23/2970) and 15 (0.64 %, 19/2970). We compared the incidence of chromosomal heteromorphisms between recurrent spontaneous abortion (RSA) group and control group. The frequency of autosomal hetermorphisms in RSA group was 7.63% higher than that in control group (5.78%), while the frequency of Y chromosomal heteromorphisms was 4.76% lower than that in control group (5.71%). Here we summarized 4 representative cases, inv (1) (p12q24), psu dic (4;17) (p16.3;p13.3), r(X)(p11; q21) and an isodicentric bisatellited chromosome to illustrate the application of C-banding or AgNOR-staining, CMA or NGS was performed to detect CNVs if necessary. Conclusions This study indicated that C-banding and AgNOR-staining were still effective complementary methods to identify chromosomal heteromorphisms and marker chromosomes or some structural rearrangements involving the centromere or acrocentric chromosomes. Our results suggested that there was no evidence for an association between chromosomal heteromorphisms and infertility or recurrent spontaneous abortions. Undoubtedly, sometimes we needed to combine the results of CMA or CNV-seq to comprehensively reflect the structure and aberration of chromosome segments. Thus, accurate karyotype reports and genetic counseling could be provided.

Three kinds of amide dendritic branches, Branch1(COOH), Branch2(COOH) and Branch3(COOH), are successfully synthesized. After mixing with diaminoctane, their self-assembly behavior in methyl methacrylate (MMA) is investigated. The structures of the amide dendritic branches are confirmed by1H-NMR and Mass spectra (MS). The gelation ability of the amide dendritic gelators is researched through tube inversion experiment, the results of which show that different structures led to quite different gelation ability, including gel-sol temperature and critical concentration to form a stable gel. SEM experiments show that three kinds of gelators formed different gel morphologies in MMA.

Gelator (G2-C12-G2) was synthesized successfully, the structure of which was confirmed by1H-NMR and Mass spectra (MS). And G2-C12-G2was used to modify PMMA by “in-situ” polymerization. The results of gel permeation chromatography (GPC) showed that Mn and Mw/Mnof the PMMA composite were barely influenced by the incorporation of gel. The results of dynamic mechanical analysis (DMA) showed that the modified PMMA had higher modulus, toughness and glass transition temperature (Tg) than pure PMMA. Furthermore the results of spectrophotometer showed that the transparency of modified PMMA was nearly the same as pure PMMA .

Ni50-xMn37In13Cox(x=0,5) alloy powders were obtained by ball-milling from the corresponding ribbon precursors. The as-milled Ni50Mn37In13and Ni45Mn37In13Co5powders show disorderedfctandfccstructures respectively, due to the larger lattice distortion in Ni45Mn37In13Co5. DSC and XRD results show that the high-temperature annealing will lead to a one-step ordering process fromfctto Heusler structure in Ni50Mn37In13, and a two-step ordering process, includingfcctobccandbccto Heusler phase transitions in Ni45Mn37In13Co5. After annealed at 400°C and 650°C, the martensitic transformation behavior is gradually and partially restored in Ni50Mn37In13powders. As modulated by the annealing temperatures, the martensitic transformation temperatures increase with the grain sizes of Ni50Mn37In13powders. However, the martensitic transformation is almost completely suppressed in 650°C annealed Ni45Mn37In13Co5powders with only some weak reflection peaks from 7M martensitic phase observed in XRD pattern.

Purpose To quantify and analyze the anterior chamber (AC) and angle (ACA) characteristics in Chinese children with different refractive status by swept-source optical coherence tomography (SS-OCT). Methods In a cross-sectional observational study, 383 children from two primary schools in Shandong Province, China, underwent a complete ophthalmic examination. First, the anterior chamber depth (ACD), anterior chamber width (ACW), angle-opening distance (AOD), and trabecular-iris space area (TISA) were evaluated automatically using a CASIA2 imaging device. AOD and TISA were measured at 500, 750µm nasal (N1 and N2, respectively), and temporal (T1 and T2, respectively) to the scleral spur (SS). Cycloplegic refraction and axial length (AL) were then measured. According to spherical equivalent refraction (SER), the children were assigned to hyperopic (SER > 0.50D), emmetropic (-0.50D < SER ≤ 0.50D), and myopic groups (SER ≤ -0.50D). Results Out of the 383 children, 349 healthy children (160 girls) with a mean age of 8.23 ± 1.06 years (range: 6–11 years) were included. The mean SER and AL were − 0.10 ± 1.57D and 23.44 ± 0.95mm, respectively. The mean ACD and ACW were 3.17 ± 0.24mm and 11.69 ± 0.43mm. The mean AOD were 0.72 ± 0.25, 0.63 ± 0.22mm at N1, T1, and 0.98 ± 0.30, 0.84 ± 0.27mm at N2, T2. The mean TISA were 0.24 ± 0.09, 0.22 ± 0.09mm2 at N1, T1, and 0.46 ± 0.16, 0.40 ± 0.14mm2 at N2, T2. The myopic group had the deepest AC and the widest angle. Compared with boys, girls had shorter AL, shallower ACD, narrower ACW, and ACA (all p < 0.05). By Pearson’s correlation analysis, SER was negatively associated with ACD, AOD, and TISA. AL was positively associated with ACD, ACW, AOD, and TISA. In the multiple regression analysis, AOD and TISA were associated with deeper ACD, narrower ACW, and longer AL. Conclusion In primary school students, the myopic eyes have deeper AC and wider angle. ACD, ACW, AOD, and TISA all increase with axial elongation. ACA is highly correlated with deeper ACD.