No abstract

The objectives of this study were to evaluate meat colour, antioxidant performance of longissimus muscle in different pig breeds, including five Chinese native pig breeds, a Chinese bred pig breed as well as a commercial crossbred pig breed, and to analyse the relationships between meat colour and antioxidant capacity. The results showed that the Chinese native and bred pig breeds have higher meat colour and marbling scores than commercial crossbred pigs. The Chinese native and bred pig breeds showed significantly stronger total antioxidant capacity and superoxide dismutase activity, and lower malondialdehyde contents than commercial crossbred pigs. Moreover, the total antioxidant capacity, superoxide dismutase activity were positively correlated with visual colour, a* and chroma, and negatively correlated with L* and hue angle; on the contrary, the malondialdehyde content was negatively correlated with visual colour, marbling, a*, b* and chroma, and positively correlated with L* and hue angle. In addition, principal component 1 and 2 explained 46.41% and 18.30% of the total variance. These results indicated that meat colour and antioxidant capacity were affected by pig breeds, and meat colour was strongly correlated with antioxidant capacity.

No abstract

No abstract

Phosphotyrosine interaction domain 1 (PID1), a protein with a phosphotyrosinebinding (PTB) domain, interacts with the lipoprotein receptor-related protein 1 (LRP1) to reduce the insulin sensitivity of adipocyte. Considering the role of LRP1 in lipid metabolism, we investigated the effect of PID1 on the content and biological activities of serum lipoproteins in pigs. PID1-transgenic pigs were genetated by sperm and magnetic nanoparticles-mediated method. The levels of PID1 in PID1-transgenic pig's liver were higher than that in the wild-type pig's liver. We found that serum levels of highdensity lipoprotein cholesterol (HDL-C) and apolipoprotein A-I (apoA-I) were significantly reduced in PID1-transgenic pigs. On the other hand, PID1-transgenic pigs displayed increased non-HDL-C levels. Serum levels of total cholesterol and triglycerides were comparable between the PID1-transgenic and the wild-type pigs. Further, the HDL isolated from PID1-transgenic pigs showed a significant reduction in cholesterol efflux ability. In addition, serum superoxide dismutase activity of PID1-transgenic pigs was also obviously lowered compared with that of wild type pigs. In conclusion, these results suggest that PID1 might be able to adjust HDL-C levels in serum and HDL cholesterol efflux ability.Abbreviations: HDL-C, high-density lipoprotein cholesterol; LCAT, Lecithin-cholesterol acyltransferase; LDL-C, low density lipoprotein cholesterol; LRP1, lipoprotein receptor-related protein 1; PID1, Phosphotyrosine interaction domain 1; SOD, serum superoxide dismutase; TC, total cholesterol; TG, triglycerides; VLDL, very low-density lipoprotein.