To study the effect of Huangzhi oral liquid (HZOL) on I/R after 2 h and 4 h and determine its regulatory function on caspase-3 and protein networks. 70 SD male rats were randomly divided into seven groups and established myocardial I/R injury model by ligating the left anterior descending coronary artery. Myocardial infarction model was defined by TTC staining and color of the heart. The levels of CK-MB, CTnI, C-RPL, SOD, and MDA were tested at 2 h and 4 h after reperfusion. HE staining and ultramicrostructural were used to observe the pathological changes. The apoptotic index (AI) of cardiomyocyte was marked by TUNEL. The expression levels of caspase-3, p53, fas, Bcl-2, and Bax were tested by immunohistochemistry and western blot. HZOL corrected arrhythmia, improved the pathologic abnormalities, decreased CK-MB, CTnI, C-RPL, MDA, AI, caspase-3, p53, fas, and Bax, and increased SOD ans Bcl-2 with different times of myocardial reperfusion; this result was similar to the ISMOC (P > 0.05). HZOL could inhibit arrhythmia at 2 and 4 h after I/R and ameliorate cardiac function, which was more significant at 4 h after reperfusion. This result may be related to decreased expression of caspase-3, p53, and fas and increased Bcl-2/Bax ratio.

We were able to demonstrate type I1 collagen in synovial phagocytes of osteoarthritis (OA) and rheumatoid arthritis (RA) patients, using a monoclonal antibody to human type I1 collagen and immunoperoxidase staining. In addition, using immunoelectron microscopy, we demonstrated labeled fragments in synovial phagocytes of both RA and OA patients. This immunohistoehemical assay may prove to be a sensitive indicator of cartilage erosion in patients with OA and RA.There is currently no sensitive indicator of active cartilage destruction in patients with arthritis. Radiographs provide only a historical record of severe damage. Clinical assessment of osteoarthritis (OA) and rheumatoid arthritis (RA) is also often inadequate; subjective assessment of pain is often the measurement used. Laboratory tests, such as erythrocyte sedimentation rate (ESR) and C-reactive protein values, are indirect measures of joint inflammation. In an effort to find markers for cartilage and joint destruction in various forms of connective tissue diseases, numerous investigators have studied bone and cartilage components, such as hydroxyproline (l), pyridinoline (2), type 111 procollagen peptide (3), proteoglycans (4), and cartilage matrix glycoprotein (3, in serum and urine. However, none of these investigations has thus far led to wider clinical use and application.Of the 5 main components of cartilage, i.e., collagen, proteoglycans, water, noncollagenous proteins, and cells, type I1 collagen makes up about half the dry weight of adult articular cartilage (6). Based on the fact that normal synovial fluid (SF) does not contain collagen, the existence of type I1 collagen in SF indicates either current ongoing or recent degradation of articular cartilage. With this in mind, we sought to detect type I1 collagen in synovial phagocytes and SF of patients with RA and OA using a monoclonal antibody (MAb) directed against human type I1 collagen. The ability to identify type I1 collagen in SF may be helpful not only in evaluating the extent of initial joint damage, but also in following the progression of joint disease.

The computerize numerical control (CNC) system is one of the key factors for the development of micro v-groove machine tools to fabrication micro v-groove structures. Based on the investigation on the machining of micro v-groove structures, a developed software contains five modules: editing, automatic programming, program control, manual control and human machine interface. A3200 software based motion controller integrated with the machine characteristics is used in this study to develop the motion control functions of the micro v-groove machine tool. Meanwhile, a method is proposed to redefine the instructions of M, T and S by setting monitoring points so as to enhance the scalability of CNC system. The results show that the CNC system software is not only stable and reliable, but also satisfies the basic requirements of the ultra-precision machining of micro v-groove structures.