The open-cut tunnels in Aixi Lake, Nanchang, are constructed in a condominium form of highway tunnel and subway tunnel, which are the first superimposed double-level tunnels in China. The scientific problems faced by this new structure form have attracted the attention of engineers and researchers. If this structure form can be reasonably used, it can reduce the repeated excavation of the ground and reduce the impact of highway tunnel construction on subway tunnels. It is of great guiding significance to study the influence of the spacing between lower double-track subway tunnels on the stress and deformation characteristics of the upper highway tunnel. Based on the model test, the deformation, axial force, bending moment, and earth pressure of the upper highway tunnel are studied. The results show that the maximum displacement of upper tunnel floor can be reduced by 30% when the spacing of the lower tunnel makes the center line of the lower single tunnel coincide with the quadripartite line of the upper tunnel. Horizontal spacing between lower double-track tunnels significantly influences the bending moment of upper tunnel floor but hardly influences the axial force of the upper tunnel floor. Considering the influence of lower tunnel spacing on the earth pressure of the upper tunnel floor, the spacing of the lower tunnel should not exceed 50% of the section width of the upper tunnel.